Rabbit Anti-PON1 Polyclonal Antibody

Référence HPA001610

Conditionnement : 100ul

Marque : Atlas Antibodies

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HPA001610
paraoxonase 1

Anti-PON1 Antibody

Polyclonal Antibody against HUMAN PON1

0.1 mg/ml
info@biovalley.fr

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Product details

  • Target protein: paraoxonase 1
  • Target gene: PON1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000002588 (78%)RAT - ENSRNOG00000008902 (76%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: A01116
  • Concentration: 0.1 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: paraoxonase 1
  • Gene name: PON1
  • Alternative gene names: ESAPON
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JMProteome Sci , 2011 Nov 17; 9:73. Epub 2011 Nov 172011 Nov 17

  • Lescuyer P, Pernin A, Hainard A, Bigeire C, Burgess JA, Zimmermann-Ivol C, Sanchez JC, Schifferli JA, Hochstrasser DF, Moll SProteomics Clin Appl , 2008 Jul; 2(7-8):1008-18. Epub 2008 Jul 102008 Jul

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