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> Rabbit Anti-TET3 Polyclonal Antibody

Rabbit Anti-TET3 Polyclonal Antibody

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Référence HPA050845-25ul

Conditionnement : 25ul

Marque : Atlas Antibodies

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HPA050845
tet methylcytosine dioxygenase 3

Anti-TET3 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN TET3

0.1 mg/ml
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
Anti-TET3 Antibody
info@biovalley.fr

Immunohistochemical staining of human placenta shows moderate nuclear positivity in a subset of cells in chorionic villi.

Product details

  • Target protein: tet methylcytosine dioxygenase 3
  • Target gene: TET3
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000034832 (91%)RAT - ENSRNOG00000011387 (88%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: C115527
  • Concentration: 0.1 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunohistochemistry (IHC) 
  • Immunofluorescence in Cell Lines (ICC-IF) 

Target information

  • Protein name: tet methylcytosine dioxygenase 3
  • Gene name: TET3
  • Alternative gene names: hCG_40738, MGC22014, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (2)

  • Qiu Z, Lin AP, Jiang S, Elkashef SM, Myers J, Srikantan S, Sasi B, Cao JZ, Godley LA, Rakheja D, Lyu Y, Zheng S, Madesh M, Shiio Y, Dahia PL, Aguiar RCCell Chem Biol , 2020 Feb 25; 27(5):538-550.e7. Epub 2020 Feb 252020 Feb 25
  • This antibody has been used for staining of 44 normal human tissue samples as well as human cancer samples covering the 20 most common cancer types and up to 12 patients for each cancer type. The results are part of an ongoing effort to map the human proteome using antibodies.
    Human Protein Atlas