Rabbit Ghrelin (GHRL) ELISA Kit
Référence MBS2025035-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Ghrelin (GHRL) ELISA Kit for Rabbit
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025035SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and… More details
Product Overview
Online Datasheet in PDFProduct Name
Ghrelin (GHRL), ELISA Kit
Full Product Name
Rabbit Ghrelin (GHRL) ELISA Kit
Product Gene Name
GHRL elisa kit
Product Synonym Names
MTLRP; Growth Hormone-Releasing Peptide
Species Reactivity
Rabbit
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
123.5-10,000pg/mL
Sensitivity
52.3pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL).
No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
9,972 Da
OMIM
605353
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Product Categories/Family for GHRL elisa kit
Signal transduction; Metabolic pathway; Endocrinology; Gastroenterology; Hormone metabolism
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of GHRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GHRL purchase
MBS2025035 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin (GHRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GHRL. The ELISA analytical biochemical technique of the MBS2025035 kit is based on GHRL antibody-GHRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
NCBI Official Full Name
ghrelin
NCBI Official Synonym Full Names
ghrelin/obestatin prepropeptide
NCBI Official Symbol
GHRL
NCBI Official Synonym Symbols
MTLRP
UniProt Protein Name
Appetite-regulating hormone
UniProt Synonym Protein Names
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46
Protein Family
Appetite-regulating hormone
UniProt Gene Name
GHRL
UniProt Synonym Gene Names
MTLRP; Ghrelin
UniProt Entry Name
GHRL_HUMAN
NCBI Protein Information
appetite-regulating hormone
NCBI Summary for GHRL
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
Show more UniProt Comments for GHRL
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach
Protein type: Apoptosis; Secreted, signal peptide; Cell development/differentiation; Cell cycle regulation; Secreted
Chromosomal Location of Human Ortholog: 3p26-p25
Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region
Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity
Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis
Disease: Obesity
Show more Protein type: Apoptosis; Secreted, signal peptide; Cell development/differentiation; Cell cycle regulation; Secreted
Chromosomal Location of Human Ortholog: 3p26-p25
Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region
Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity
Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; positive regulation of multicellular organism growth; decidualization; positive regulation of adrenocorticotropic hormone secretion; negative regulation of insulin secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis
Disease: Obesity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with GHRL elisa kit
| Reference Product | PubMed Publications |
|---|---|
| GHSR elisa kit | >28 publications with GHRL and GHSR |
| NPY elisa kit | >12 publications with GHRL and NPY |
| LEP elisa kit | >11 publications with GHRL and LEP |
| CCK elisa kit | >5 publications with GHRL and CCK |
| GHRH elisa kit | >3 publications with GHRL and GHRH |
| AGRP elisa kit | >3 publications with GHRL and AGRP |
| GCG elisa kit | >2 publications with GHRL and GCG |
| IGF1 elisa kit | >1 publications with GHRL and IGF1 |
| GPR39 elisa kit | >1 publications with GHRL and GPR39 |
Pathways associated with GHRL elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies | Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram |
| Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies | Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram |
| Metabolism Of Proteins Pathway antibodies | Metabolism Of Proteins Pathway Diagram |
| Peptide Hormone Metabolism Pathway antibodies | Peptide Hormone Metabolism Pathway Diagram |
Diseases associated with GHRL elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Obesity Antibodies | >52 publications with GHRL and Obesity |
| Insulin Resistance Antibodies | >18 publications with GHRL and Insulin Resistance |
| Nervous System Diseases Antibodies | >13 publications with GHRL and Nervous System Diseases |
| Weight Gain Antibodies | >12 publications with GHRL and Weight Gain |
| Inflammation Antibodies | >10 publications with GHRL and Inflammation |
| Cachexia Antibodies | >6 publications with GHRL and Cachexia |
| Kidney Diseases Antibodies | >5 publications with GHRL and Kidney Diseases |
| Heart Diseases Antibodies | >5 publications with GHRL and Heart Diseases |
| Breast Neoplasms Antibodies | >5 publications with GHRL and Breast Neoplasms |
| Necrosis Antibodies | >5 publications with GHRL and Necrosis |
Organs/Tissues associated with GHRL elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >78 publications with GHRL and Blood |
| Stomach Antibodies | >27 publications with GHRL and Stomach |
| Brain Antibodies | >23 publications with GHRL and Brain |
| Connective Tissue Antibodies | >11 publications with GHRL and Connective Tissue |
| Intestine Antibodies | >4 publications with GHRL and Intestine |
| Prostate Antibodies | >2 publications with GHRL and Prostate |
| Embryonic Tissue Antibodies | >1 publications with GHRL and Embryonic Tissue |
| Lung Antibodies | >1 publications with GHRL and Lung |