Rabbit polyclonal antibody to ERK5

Référence CPA9284-30ul

Conditionnement : 30ul

Marque : Cohesion Biosciences

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  • Western blot analysis of ERK5 expression in Hela (A), 293T (B), NIH3T3 (C), mouse skeletal muscle (D), rat kidney (E), rat skeletal muscle (F) whole cell lysates.
  • Immunohistochemical analysis of ERK5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-ERK5 Antibody
Cat No: CPA9284
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Description: Rabbit polyclonal antibody to ERK5
Immunogen: Recombinant protein corresponding to human ERK5.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500)
Gene Symbol: MAPK7
Alternative Names: BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; MAP kinase 7; MAPK 7; Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5
Entrez Gene (Human):
SwissProt (Human):
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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Référence
Description
Cond.
Prix HT
AR0135-02
 0.22um,9cm×10cm;20sheets