Rat alpha-Smooth muscle actin (alpha-SMA) ELISA Kit
Référence MBS266620-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Alpha-Smooth Muscle Actin (alpha-SMA) ELISA Kit for Rat
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS266620Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat alpha-SMA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
alpha-Smooth muscle actin (alpha-SMA), ELISA Kit
Full Product Name
Rat alpha-Smooth muscle actin (alpha-SMA) ELISA Kit
Product Gene Name
alpha-SMA elisa kit
Product Synonym Names
Rat a-Smooth muscle actin (a-SMA) ELISA Kit
Species Reactivity
Rat
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Inter-assay Precision
<= 12%
Intra-assay Precision
<= 8%
Molecular Weight
37,083 Da
OMIM
102545
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of alpha-SMA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for alpha-SMA purchase
MBS266620 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-Smooth muscle actin (alpha-SMA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha-SMA. The ELISA analytical biochemical technique of the MBS266620 kit is based on alpha-SMA antibody-alpha-SMA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha-SMA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, alpha-SMA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for alpha-SMA. It may not necessarily be applicable to this product.
NCBI Official Full Name
actin, gamma-enteric smooth muscle isoform 1
NCBI Official Synonym Full Names
actin, gamma 2, smooth muscle, enteric
NCBI Official Symbol
ACTG2
NCBI Official Synonym Symbols
ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
UniProt Protein Name
Actin, gamma-enteric smooth muscle
UniProt Synonym Protein Names
Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin
UniProt Gene Name
ACTG2
UniProt Synonym Gene Names
ACTA3; ACTL3; ACTSG
UniProt Entry Name
ACTH_HUMAN
NCBI Protein Information
actin, gamma-enteric smooth muscle; alpha-actin-3; actin-like protein
NCBI Summary for alpha-SMA
Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]
Show more UniProt Comments for alpha-SMA
ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.
Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: extracellular space; cytoskeleton; cytosol
Molecular Function: ATP binding
Biological Process: muscle contraction
Disease: Visceral Myopathy
Show more Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p13.1
Cellular Component: extracellular space; cytoskeleton; cytosol
Molecular Function: ATP binding
Biological Process: muscle contraction
Disease: Visceral Myopathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with alpha-SMA elisa kit
| Reference Product | PubMed Publications |
|---|---|
| ACTA2 elisa kit | >2 publications with alpha-SMA and ACTA2 |
| TAGLN elisa kit | >2 publications with alpha-SMA and TAGLN |
| CNN1 elisa kit | >1 publications with alpha-SMA and CNN1 |
Pathways associated with alpha-SMA elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Muscle Contraction Pathway antibodies | Muscle Contraction Pathway Diagram |
| Smooth Muscle Contraction Pathway antibodies | Smooth Muscle Contraction Pathway Diagram |
| Vascular Smooth Muscle Contraction Pathway antibodies | Vascular Smooth Muscle Contraction Pathway Diagram |
| Vascular Smooth Muscle Contraction Pathway antibodies | Vascular Smooth Muscle Contraction Pathway Diagram |
Diseases associated with alpha-SMA elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Adenocarcinoma Antibodies | >3 publications with alpha-SMA and Adenocarcinoma |
| Liver Neoplasms Antibodies | >3 publications with alpha-SMA and Liver Neoplasms |
| Neoplasm Metastasis Antibodies | >3 publications with alpha-SMA and Neoplasm Metastasis |
| Liver Diseases Antibodies | >2 publications with alpha-SMA and Liver Diseases |
| Megacystis microcolon intestinal hypoperistalsis syndrome Antibodies | >2 publications with alpha-SMA and Megacystis microcolon intestinal hypoperistalsis syndrome |
| Anemia Antibodies | >1 publications with alpha-SMA and Anemia |
| Disease Models, Animal Antibodies | >1 publications with alpha-SMA and Disease Models, Animal |
| Carcinoma, Hepatocellular Antibodies | >1 publications with alpha-SMA and Carcinoma, Hepatocellular |
| Lung Neoplasms Antibodies | >1 publications with alpha-SMA and Lung Neoplasms |
| Neoplasms, Experimental Antibodies | >1 publications with alpha-SMA and Neoplasms, Experimental |
Organs/Tissues associated with alpha-SMA elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Muscle Antibodies | >10 publications with alpha-SMA and Muscle |
| Liver Antibodies | >3 publications with alpha-SMA and Liver |
| Lung Antibodies | >3 publications with alpha-SMA and Lung |
| Intestine Antibodies | >3 publications with alpha-SMA and Intestine |
| Bladder Antibodies | >2 publications with alpha-SMA and Bladder |
| Connective Tissue Antibodies | >2 publications with alpha-SMA and Connective Tissue |
| Kidney Antibodies | >1 publications with alpha-SMA and Kidney |
| Embryonic Tissue Antibodies | >1 publications with alpha-SMA and Embryonic Tissue |
| Testis Antibodies | >1 publications with alpha-SMA and Testis |
| Bone Antibodies | >1 publications with alpha-SMA and Bone |
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