Rat Alpha-type platelet-derived growth factor receptor (PDGFRA) ELISA Kit

Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDGFRA in samples. An antibody specific for PDGFRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGFRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFRA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-type platelet-derived growth factor receptor is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.