G-Series Rat Cytokine Array 3 Kit. Semi-quantitative measurement of 27 rat cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 16 Sample Kit, 32 Sample Kit, 64 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 27 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).
Product Features
- High sensitivity (pg/mL to ng/mL levels)
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Test 16 samples on each slide
- Data analysis software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
beta-NGF
| CD86 (B7-2)
| CINC-1 (CXCL1)
| CINC-2
| CINC-3
|
CNTF
| Fractalkine (CX3CL1)
| GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-13
| IL-2
|
IL-4
| IL-6
| LIX
| L-Selectin (CD62L)
| MCP-1 (CCL2)
|
PDGF-AA
| Prolactin R
| RAGE
| TCK-1 (CXCL7)
| TIMP-1
|
TNF alpha
| VEGF-A
| |||
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Detection of Relative Protein Expression
- Detecting Patterns of Cytokine Expression
- Biomarker Screening
- Identifying Key Factors
- Confirming a Biological Process
Kit Components
- Antibody Array Slide/s
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with samples
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAR-CYT-3.gal)
Excel (GSR-CYT-3_Analysis_Tool.xlsx)
citations
Engineering functional vasculatures to reconstruct sympathetic-parasympathetic circuits for bladder function after spinal cord injury
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (supernatant of spinal cord injury site tissues)
- Year:
- 2027
- Journal Name:
- Bioactive Materials
- PubMed ID:
- 42571573
Reduction of alternative polarization of macrophages by short-term activated hepatic stellate cell-derived small extracellular vesicles
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (rat KC kupffer cell culture media)
- Year:
- 2025
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
- PubMed ID:
- 40211350
Effects and mechanisms of breastmilk stem cells in the treatment of white matter injury in newborn rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (CC tissues)
- Year:
- 2025
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 40055828
Establishment of fracture blister model and analysis of plasma protein markers in rats
- Year:
- 2025
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 40297588
Anisotropic microtopography surface of chitosan scaffold regulating skin precursor-derived Schwann cells towards repair phenotype promotes neural regeneration
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (SKP-SC conditioned medium)
- Year:
- 2024
- Journal Name:
- Regenerative Biomaterials
- PubMed ID:
- 38414797
Effects of xenogeneic transplantation of umbilical cord-derived mesenchymal stem cells combined with irbesartan on renal podocyte damage in diabetic rats
- Species:
- Rat
- Sample Type:
- Other / Unspecified (renal cortex)
- Year:
- 2024
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 39080783
Dracorhodin perchlorate alleviates sciatic nerve pain in CCI rats by modulating inflammation and promoting nerve repair
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (protein samples sciatic nerve tissue)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 41361206
Switching On and Off Macrophages by a “BridgeBurning†Coating Improves BoneImplant Integration under Osteoporosis
- Species:
- Rat
- Sample Type:
- Conditioned media (Cell culture media)
- Year:
- 2020
Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (rat nerve tissue)
- Year:
- 2021
- Journal Name:
- Neural Regeneration Research
- PubMed ID:
- 33433493
Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (spinal cord tissue protein extracts)
- Year:
- 2020
- Journal Name:
- Theranostics
- PubMed ID:
- 33391526



