Rat Cytokine Array GS3 (Glass Slide)

Référence GSR-CYT-3-4

Conditionnement : 64SampleKit

Marque : RayBiotech

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Rat Cytokine Array GS3

G-Series Rat Cytokine Array 3 Kit. Semi-quantitative measurement of 27 rat cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesRat
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected27
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice
Storage-20°C

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
beta-NGF
  • Gene Symbol: NGF
  • Accession #: P25427
  • ELISA Kit: RayBio Rat beta-NGF ELISA
CD86
(B7-2)
  • Gene Symbol: CD86
  • Accession #: O35531
  • ELISA Kit: RayBio Rat CD86 ELISA
CINC-1
(CXCL1)
  • Gene Symbol: CXCL1
  • Accession #: P14095
  • ELISA Kit: RayBio Rat CINC-1 ELISA
CINC-2
  • Gene Symbol: CXCL3
  • Accession #: Q10746
  • ELISA Kit: RayBio Rat CINC-2 ELISA
CINC-3
  • Gene Symbol: Cxcl2
  • Accession #: P30348
  • ELISA Kit: RayBio Rat CINC-3 ELISA
CNTF
  • Gene Symbol: CNTF
  • Accession #: P20294
  • ELISA Kit: RayBio Rat CNTF ELISA
Fractalkine
(CX3CL1)
  • Gene Symbol: CX3CL1
  • Accession #: Q6IRF7
  • ELISA Kit: RayBio Rat Fractalkine ELISA
GM-CSF
  • Gene Symbol: CSF2
  • Accession #: P48750
  • ELISA Kit: RayBio Rat GM-CSF ELISA
ICAM-1
(CD54)
  • Gene Symbol: ICAM1
  • Accession #: Q00238
  • ELISA Kit: RayBio Rat ICAM-1 ELISA
IFN-gamma
  • Gene Symbol: IFNG
  • Accession #: P01581
  • ELISA Kit: RayBio Rat IFN-gamma ELISA
IL-1 alpha
(IL-1 F1)
  • Gene Symbol: IL1A
  • Accession #: P16598
  • ELISA Kit: RayBio Rat IL-1 alpha ELISA
IL-1 beta
(IL-1 F2)
  • Gene Symbol: IL1B
  • Accession #: Q63264
  • ELISA Kit: RayBio Rat IL-1 beta ELISA
IL-10
  • Gene Symbol: IL10
  • Accession #: P29456
  • ELISA Kit: RayBio Rat IL-10 ELISA
IL-13
  • Gene Symbol: IL13
  • Accession #: P42203
  • ELISA Kit: RayBio Rat IL-13 ELISA (For Lysates)
IL-2
  • Gene Symbol: IL2
  • Accession #: P17108
  • ELISA Kit: RayBio Rat IL-2 ELISA
IL-4
  • Gene Symbol: IL4
  • Accession #: P20096
  • ELISA Kit: RayBio Rat IL-4 ELISA
IL-6
  • Gene Symbol: IL6
  • Accession #: P20607
  • ELISA Kit: RayBio Rat IL-6 ELISA
LIX
  • Gene Symbol: Cxcl5
  • Accession #: P97885
  • ELISA Kit: RayBio Rat LIX ELISA
L-Selectin
(CD62L)
  • Gene Symbol: SELL
  • Accession #: P30836
  • ELISA Kit: RayBio Rat L-Selectin ELISA
MCP-1
(CCL2)
  • Gene Symbol: CCL2
  • Accession #: P14844
  • ELISA Kit: RayBio Rat MCP-1 ELISA
PDGF-AA
  • Gene Symbol: PDGFA
  • Accession #: Q6P7C3
  • ELISA Kit: RayBio Rat PDGF-AA ELISA
Prolactin R
  • Gene Symbol: Prlr
  • Accession #: P05710
  • ELISA Kit: RayBio Rat Prolactin R ELISA
RAGE
  • Gene Symbol: MOK
  • Accession #: Q63495
  • ELISA Kit: RayBio Rat RAGE ELISA
TCK-1
(CXCL7)
  • Gene Symbol: Ppbp
  • Accession #: Q99ME0
  • ELISA Kit: RayBio Rat TCK-1 ELISA
TIMP-1
  • Gene Symbol: TIMP1
  • Accession #: P30120
  • ELISA Kit: RayBio Rat TIMP-1 ELISA
TNF alpha
  • Gene Symbol: TNF
  • Accession #: P16599
  • ELISA Kit: RayBio Rat TNF-alpha ELISA
VEGF-A
  • Gene Symbol: VEGFA
  • Accession #: P16612
  • ELISA Kit: RayBio Rat VEGF-A ELISA

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAR-CYT-3.gal)
Excel (GSR-CYT-3_Analysis_Tool.xlsx)

citations

  • Engineering functional vasculatures to reconstruct sympathetic-parasympathetic circuits for bladder function after spinal cord injury

    Species:
    Rat
    Sample Type:
    Cell Culture Supernatant (supernatant of spinal cord injury site tissues)
    Year:
    2027
    Journal Name:
    Bioactive Materials
    PubMed ID:
    42571573
  • Reduction of alternative polarization of macrophages by short-term activated hepatic stellate cell-derived small extracellular vesicles

    Species:
    Rat
    Sample Type:
    Cell Culture Supernatant (rat KC kupffer cell culture media)
    Year:
    2025
    Journal Name:
    Journal of Experimental & Clinical Cancer Research
    PubMed ID:
    40211350
  • Effects and mechanisms of breastmilk stem cells in the treatment of white matter injury in newborn rats

    Species:
    Rat
    Sample Type:
    Tissue Lysate / Homogenate (CC tissues)
    Year:
    2025
    Journal Name:
    Stem Cell Research & Therapy
    PubMed ID:
    40055828
  • Establishment of fracture blister model and analysis of plasma protein markers in rats

    Year:
    2025
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    40297588
  • Anisotropic microtopography surface of chitosan scaffold regulating skin precursor-derived Schwann cells towards repair phenotype promotes neural regeneration

    Species:
    Rat
    Sample Type:
    Cell Culture Supernatant (SKP-SC conditioned medium)
    Year:
    2024
    Journal Name:
    Regenerative Biomaterials
    PubMed ID:
    38414797
  • Effects of xenogeneic transplantation of umbilical cord-derived mesenchymal stem cells combined with irbesartan on renal podocyte damage in diabetic rats

    Species:
    Rat
    Sample Type:
    Other / Unspecified (renal cortex)
    Year:
    2024
    Journal Name:
    Stem Cell Research & Therapy
    PubMed ID:
    39080783
  • Dracorhodin perchlorate alleviates sciatic nerve pain in CCI rats by modulating inflammation and promoting nerve repair

    Species:
    Rat
    Sample Type:
    Tissue Lysate / Homogenate (protein samples sciatic nerve tissue)
    Year:
    2025
    Journal Name:
    Scientific Reports
    PubMed ID:
    41361206
  • Switching On and Off Macrophages by a “BridgeBurning” Coating Improves BoneImplant Integration under Osteoporosis

    Species:
    Rat
    Sample Type:
    Conditioned media (Cell culture media)
    Year:
    2020
  • Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury

    Species:
    Rat
    Sample Type:
    Tissue Lysate / Homogenate (rat nerve tissue)
    Year:
    2021
    Journal Name:
    Neural Regeneration Research
    PubMed ID:
    33433493
  • Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist

    Species:
    Rat
    Sample Type:
    Tissue Lysate / Homogenate (spinal cord tissue protein extracts)
    Year:
    2020
    Journal Name:
    Theranostics
    PubMed ID:
    33391526