Rat Cytokine Array GS7 (Glass Slide)

Référence GSR-CYT-7-5

Conditionnement : 64SampleKit

Marque : RayBiotech

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Rat Cytokine Array GS7

G-Series Rat Cytokine Array 7 Kit. Semi-quantitative measurement of 40 rat cytokines. Suitable for all liquid sample types.

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Product Description

Specifications

Size16 Sample Kit, 32 Sample Kit, 64 Sample Kit
SpeciesRat
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-QuantitativeSemi-Quantitative
Number of Targets Detected40
Solid Support
Glass Slide
Design PrincipleSandwich-based
Method Of DetectionFluorescence Laser Scanner
Shipping TypeBlue ice

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

Scroll over each target protein for more information
IL-9
  • Gene Symbol: Il9 rCG_24215
  • Gene ID: 116558
  • Accession #: D4A8I9
  • ELISA Kit: RayBio Rat IL-9 ELISA
IL-9 R
(CD129)
  • Gene Symbol: Il9r rCG_32550
  • Accession #: G3V8S1
  • ELISA Kit: RayBio Rat IL-9 R ELISA
Jagged 1
  • Gene Symbol: Jag1
  • Gene ID: 29146
  • Accession #: Q63722
  • ELISA Kit: RayBio Rat Jagged 1 ELISA
JAM-C
(CD323)
  • Gene Symbol: Jam3
  • Gene ID: 315509
  • Accession #: Q68FQ2
  • ELISA Kit: RayBio
KDR
  • Gene Symbol: Kdr Flk1
  • Gene ID: 25589
  • Accession #: O08775
  • ELISA Kit: RayBio Rat VEGFR2/KDR ELISA
KLKB1
  • Gene Symbol: Klkb1 Klk3 Pk
  • Accession #: P14272
  • ELISA Kit: RayBio
Klotho beta
  • Gene Symbol: Kl
  • Gene ID: 83504
  • Accession #: Q9Z2Y9
  • ELISA Kit: RayBio
Lactoferrin
  • Gene Symbol: Ltf Ltf_predicted rCG_25565
  • Gene ID: 301034
  • Accession #: D3ZAB1
  • ELISA Kit: RayBio
Layilin
  • Gene Symbol: Layn
  • Gene ID: 500996
  • Accession #: D3Z895
  • ELISA Kit: RayBio Rat Layilin ELISA
Legumain
  • Gene Symbol: Lgmn Prsc1
  • Gene ID: 63865
  • Accession #: Q9R0J8
  • ELISA Kit: RayBio
Leptin
  • Gene Symbol: Lenep Lep503
  • Gene ID: 113917
  • Accession #: Q9WVB7
  • ELISA Kit: RayBio Rat Leptin ELISA
LIF
LRPAP
  • Gene Symbol: Lrpap1
  • Gene ID: 116565
  • Accession #: Q99068
  • ELISA Kit: RayBio
Lumican
  • Gene Symbol: Lum Lcn Ldc
  • Gene ID: 81682
  • Accession #: P51886
  • ELISA Kit: RayBio
Lymphotoxin beta R
(TNF RIII)
  • Gene Symbol: Ltbr rCG_29964
  • Gene ID: 297604
  • Accession #: Q5U2S8
  • ELISA Kit: RayBio Rat Lymphotoxin beta R ELISA
MAG
  • Gene Symbol: Mag
  • Gene ID: 29409
  • Accession #: P07722
  • ELISA Kit: RayBio Rat MAG ELISA
Matrilin-4
  • Gene Symbol: Matn4 Matn4_predicted rCG_32351
  • Gene ID: 296358
  • Accession #: D3ZMS3
  • ELISA Kit: RayBio
MCP-3
(MARC/CCL7)
  • Gene Symbol: Ccl7 Mcp3 Scya7
  • Gene ID: 287561
  • Accession #: Q9QXY8
  • ELISA Kit: RayBio
MSP alpha/beta
  • Gene Symbol: Mst1 MSP rCG_25288
  • Gene ID: 24566
  • Accession #: P70521
  • ELISA Kit: RayBio
Nephrin
Neurexin 1 alpha
  • Gene Symbol: Nrxn1
  • Gene ID: 60391
  • Accession #: Q63372
  • ELISA Kit: RayBio Rat Neurexin-1 alpha ELISA
Neurexophilin-3
  • Gene Symbol: Nxph3 Nph3
  • Gene ID: 59315
  • Accession #: Q9Z2N5
  • ELISA Kit: RayBio
Neuroplastin
  • Gene Symbol: Nptn Sdfr1 Sdr1
  • Gene ID: 56064
  • Accession #: P97546
  • ELISA Kit: RayBio
Noggin
  • Gene Symbol: Nog rCG_34836
  • Gene ID: 25495
  • Accession #: G3V8X8
  • ELISA Kit: RayBio
Notch-3
  • Gene Symbol: Notch3
  • Gene ID: 56761
  • Accession #: Q9R172
  • ELISA Kit: RayBio
Osteopontin
(SPP1)
  • Gene Symbol: Spp1 2b7 Spp-1
  • Gene ID: 25353
  • Accession #: P08721
  • ELISA Kit: RayBio Rat OPN ELISA
OX40
(TNFRSF4)
  • Gene Symbol: Tnfrsf4 Ox40 Txgp1l
  • Gene ID: 25572
  • Accession #: P15725
  • ELISA Kit: RayBio
PDGF R alpha
  • Gene Symbol: Pdgfra
  • Gene ID: 25267
  • Accession #: P20786
  • ELISA Kit: RayBio Rat PDGF R alpha ELISA
PDGF-BB
  • Accession #:
  • ELISA Kit: RayBio Rat PDGF-BB ELISA
PGC
  • Gene Symbol: Pgc
  • Gene ID: 24864
  • Accession #: P04073
  • ELISA Kit: RayBio
pIgR
  • Gene Symbol: Pigr
  • Gene ID: 25046
  • Accession #: P15083
  • ELISA Kit: RayBio
Plexin A1
  • Gene Symbol: Plxna1 RGD1560871 rCG_56095
  • Gene ID: 362398
  • Accession #: D3Z981
  • ELISA Kit: RayBio
PLTP
  • Gene Symbol: Pltp
  • Gene ID: 296371
  • Accession #: E9PSP1
  • ELISA Kit: RayBio
Pref-1
(DLK-1/FA1)
  • Gene Symbol: Dlk1 ZOG
  • Gene ID: 114587
  • Accession #: O70534
  • ELISA Kit: RayBio
PVRL1
  • Gene Symbol: Nectin1 Pvrl1 rCG_58281
  • Gene ID: 192183
  • Accession #: F1LNP8
  • ELISA Kit: RayBio Rat PVRL1/Nectin-1 ELISA
RBP4
  • Gene Symbol: Rbp4
  • Gene ID: 25703
  • Accession #: P04916
  • ELISA Kit: RayBio Rat RBP4 ELISA
Reg3A
  • Gene Symbol: Reg3a Pap2 Reg3
  • Gene ID: 171162
  • Accession #: P35231
  • ELISA Kit: RayBio
Renin 1
  • Accession #:
  • ELISA Kit: RayBio Rat Renin-1 ELISA
RGM-B
  • Gene Symbol: Rgmb LOC681433
  • Gene ID: 681433
  • Accession #: M0RB24
  • ELISA Kit: RayBio
ROBO1
  • Gene Symbol: Robo1
  • Gene ID: 58946
  • Accession #: O55005
  • ELISA Kit: RayBio Rat ROBO1 ELISA

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

GAL (GAL-QAR-CYT-7.gal)
Excel (GSR-CYT-7_Analysis_Tool.xlsx)

citations

  • Establishment of fracture blister model and analysis of plasma protein markers in rats

    Year:
    2025
    Journal Name:
    Frontiers in Immunology
    PubMed ID:
    40297588