Quantibody® Rat Cytokine Array 3 Kit. Detects 27 Rat Cytokines. Suitable for all liquid sample types.
Rat Cytokine Array Q3
Rat Cytokine Array Q3
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Rat |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 27 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| beta-NGF
| CD86 (B7-2)
| CINC-1 (CXCL1)
| CINC-2
| CINC-3
|
| CNTF
| Fractalkine (CX3CL1)
| GM-CSF
| ICAM-1 (CD54)
| IFN-gamma
|
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-13
| IL-2
|
| IL-4
| IL-6
| LIX
| L-Selectin (CD62L)
| MCP-1 (CCL2)
|
| PDGF-AA
| Prolactin R
| RAGE
| TCK-1 (CXCL7)
| TIMP-1
|
| TNF alpha
| VEGF-A
| |||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Rat Cytokine Array Q3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAR-CYT-3.gal)
Excel (QAR-CYT-3_Analysis_Tool.xls)
citations
Thrombospondin 1 enhances systemic inflammation and disease severity in acute-on-chronic liver failure
- Species:
- Rat
- Sample Type:
- Serum (ACLF, liver cirrhosis and normal rats)
- Year:
- 2024
- Journal Name:
- BMC Medicine
- PubMed ID:
- 38439091
Network-level organization of systemic inflammation reflects early Alzheimer's-like behavioral changes
- Species:
- Rat
- Sample Type:
- Serum (WT vs TgAD rats)
- Year:
- 2025
- Journal Name:
- Scientific Reports
- PubMed ID:
- 41345217
CINC-2 and miR-199a-5p in EVs secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration
- Species:
- Rat
- Sample Type:
- mesenchymal cells (MCs)
- Year:
- 2023
- Journal Name:
- Stem Cell Research & Therapy
Pharmacological Characterization of a Novel Neuroactive Steroid Prodrug, NTS-104, and Its Neuroprotective Activity in Experimental Stroke Models
- Species:
- Rat
- Sample Type:
- cortex, plasma
- Year:
- 2022
- Journal Name:
- Stroke
Janus fibre/sponge composite combined with IOPNs promotes haemostasis and efficient reconstruction in oral guided bone regeneration
- Species:
- Rat
- Sample Type:
- Bone Mesenchymal Stem Cells (BMSC)
- Year:
- 2022
- Journal Name:
- Materials & Design
Sacubitril/valsartan inhibits obesity-associated diastolic dysfunction through suppression of ventricular-vascular stiffness
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (heart tissue)
- Year:
- 2021
- Journal Name:
- Cardiovascular Diabetology
- PubMed ID:
- 33882908
In a circuit necessary for cognition and emotional affect, Alzheimer's-like pathology associates with neuroinflammation, cognitive and motivational deficits in the young adult TgF344-AD rat
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (brain tissue punches PrL, NAc, BLA protein lysates)
- Year:
- 2024
- Journal Name:
- Brain, behavior, & immunity - health
- PubMed ID:
- 39022628
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of ovarian research
Neuroprotective potential of intranasally delivered L-myc immortalized human neural stem cells in female rats after a controlled cortical impact injury
- Species:
- Rat
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- Scientific reports
Extracellular vesicles containing miR-146a-5p secreted by bone marrow mesenchymal cells activate hepatocytic progenitors in regenerating rat livers
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (EV lysates); Extracellular Vesicles
- Year:
- 2021
- Journal Name:
- Stem Cell Research & Therapy
- PubMed ID:
- 34051870
