General Information
| Short Description | This IFN-Gamma antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Rat IFN-Gamma protien.Detection Immunogen: Recombinant Rat IFN-Gamma protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation. |
| Protein Name | Interferon GammaIfn-Gamma |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Interferon Gamma antibody pairIfn-Gamma antibody pairIfng antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat IFN-Gamma (Interferon Gamma) Antibody Pair (STJAP00594)
This antibody pair detects rat IFN-Gamma (Interferon Gamma), corresponding to UniProt entry P01581. Reactivity is confirmed for rat samples only.
The capture antibody is an unconjugated mouse-raised antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5). The detection antibody is a biotin-conjugated goat-raised antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5).
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Compatible sample types include serum, plasma, and other biological fluids from rat.
The capture antibody was purified by Protein A or G affinity chromatography, while the detection antibody was purified by antigen affinity purification. Both were raised against recombinant rat IFN-Gamma protein.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.