Rat IgA Antibody - HRP Conjugated
Référence OASA06770
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Goat Anti-Rat IgA Polyclonal Antibody - HRP Conjugated (OASA06770)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rat IgA Polyclonal Antibody - HRP Conjugated (OASA06770) |
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| Predicted Species Reactivity | Rat |
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| Product Format | Liquid. Phosphate buffered saline. |
| Clonality | Polyclonal |
| Isotype | Polyclonal IgG |
| Host | Goat |
| Conjugation | HRP |
| Application | ELISA|WB |
| :: | Preservative Stabilisers: 0.1% - Proclin 300 0.2% - Bovine Serum Albumin Antiserum Preparation: Antisera to rat IgA were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline |
| Reconstitution and Storage | 2°C to 8°C |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 1 mg/ml |
| Specificity | IgA |
| Application Info | ELISA: 1/10,000 Western Blotting: 1/1,000 - 10,000 |
| Protocol Information | Citation: 1: Matsuo R, Garrett JR, Proctor GB, Carpenter GH. Reflex secretion of proteins into submandibular saliva in conscious rats, before and after preganglionic sympathectomy. J Physiol. 2000 Aug 15;527 Pt 1:175-84. PubMed PMID: 10944180; PubMed Central PMCID: PMC2270057. Species: Rat Experiment Name: 1. Assays of salivary proteins2. Electrophoresis of salivary proteins Experiment Background: 1. The levels of SIgA present in each saliva sample were quantified by enzyme linked immunosorbent assay (ELISA).2. Composition of salivary protein resolved by electrophoresis Experimental Steps: 1. Assays of salivary proteinsThis was performed on 96well microtitre plates which were coated overnight at 4 C with rabbit anti-rat IgA ( Ltd, Oxford, UK) diluted 1 in 2000 with 0•1 M sodium carbonate buffer (pH 9•6). Plates were washed 3 times in 0•1 M phosphate buffered saline (pH 7•0) containing 0•15 M sodium chloride and 0•1% Tween 20 (PBST) followed by water and then samples were placed on the coated plates and serially diluted in PBST.Samples were incubated on the plates for 2 h at 37 C then the plates were washed as above. SIgA purified from rat bile (Carpenter et al. 1998) was quantified by a modified Lowry protein assay (Petersen, 1977) and was used as a standard for quantifying the SIgA content of samples. Horseradish peroxidaselabelled rabbit anti-rat IgA antibodies ( Ltd), diluted 1 in 2000 in PBST, were incubated on the plate for 1 h at 37 C. Following incubation, plates were washed |