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> Rat IgM (mu chain) antibody (Peroxidase)

Rat IgM (mu chain) antibody (Peroxidase)

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Conditionnement : 1mg

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    Rat IgM (mu chain) antibody (Peroxidase)

    Catalog Number: orb347812

    Catalog Numberorb347812
    CategoryAntibodies
    DescriptionRat IgM (mu chain) antibody (Peroxidase)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgM whole molecule
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
    ConjugationHRP
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Alternative namesgoat anti-Rat IgM (mu chain) Antibody peroxidase C
    NoteFor research use only
    Application notesAnti-Rat IgM Peroxidase Conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    Rat IgM (mu chain) antibody (Peroxidase)

    Western Blot of Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n orb347802). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody (p/n orb347802) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.