RayBio® Rat IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Rat IL-1 beta ELISA Kit
Rat IL-1 beta ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 80 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 80 pg/ml - 50000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | Q63264 |
| Gene Symbols | IL1B |
| Protein Name / Synonyms | Interleukin-1 beta (IL-1 beta) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
| Sample type | Cell Culture Supernatants / Plasma / Serum, Cell and Tissue Lysates |
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Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat IL-1 beta ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified IL-1 beta (IL-1F2 / IL1B), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
The potential role of mesenchymal stem cells enhanced with melatonin in renal damage induced experimentally by cecal ligation and puncture in rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (kidney homogenates)
- Year:
- 2026
- Journal Name:
- Frontiers in Immunology
- PubMed ID:
- 41798959
Multi-target antidiabetic and organ-protective effects of a polyherbal ethanol extract in STZ-induced diabetic rats
- Species:
- Rat
- Sample Type:
- Serum (STZ-diabetic wistar rats)
- Year:
- 2026
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 42453573
Neuroprotective effects of telmisartan in a harmaline-induced model of essential tremor: modulation of the renin-angiotensin system and inflammatory pathways
- Species:
- Rat
- Sample Type:
- Other / Unspecified (cerebella)
- Year:
- 2026
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 42305442
Dysregulation of protein degradation and alteration of secretome in α-synuclein-exposed astrocytes: implications for dopaminergic neuronal dysfunction
- Species:
- Rat
- Sample Type:
- Cell Culture Supernatant (conditioned media of astrocytes or DA neurons)
- Year:
- 2024
- Journal Name:
- Cell Communication and Signaling
- PubMed ID:
- 39617881
Chaperone-mediated autophagy, heat shock protein 70, and serotonin: novel targets of beta-hydroxybutyrate in HFFD/LPS-induced sporadic Alzheimer's disease model
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (hippocampus)
- Year:
- 2025
- Journal Name:
- Inflammopharmacology
- PubMed ID:
- 40319428
2-(4-Methylthiazol-5-yl) Ethyl Nitrate Hydrochloride Ameliorates Cognitive Impairment via Modulation of Oxidative Stress and Nuclear Factor Kappa B (NF-κB) Signaling Pathway in Chronic Cerebral Hypoperfusion-Associated Spontaneously Hypertensive Rats
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (cortical or hippocampal homogenate)
- Year:
- 2024
- Journal Name:
- Antioxidants
- PubMed ID:
- 38790690
Epigallocatechin-3-gallate conjugated with selenium nanoparticles prevents neurological complications in rats exhibiting schizophrenia-like behaviors
- Species:
- Rat
- Sample Type:
- Other / Unspecified (prefrontal cortex)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 41170387
Parthanatos drives cognitive decline in repeated brain trauma: MSC-derived exosomes as a novel therapeutic strategy
- Species:
- Rat
- Sample Type:
- Tissue Lysate / Homogenate (cortical tissue)
- Year:
- 2025
- Journal Name:
- Frontiers in Pharmacology
- PubMed ID:
- 40963678
Melatonin promotes gut anti-oxidative status in perinatal rat by remodeling the gut microbiome
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Redox biology
Disease-modifying effects of a glial-targeted inducible nitric oxide synthase inhibitor (1400W) in mixed-sex cohorts of a rat soman (GD) model of epilepsy
- Species:
- Rat
- Sample Type:
- Serum, cerebrospinal fluid (CSF)
- Year:
- 2023
- Journal Name:
- Journal of Neuroinflammation