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Rat Insulin ELISA Kit
Rat Insulin ELISA Kit
RayBio® Rat Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Rat |
| Sensitivity | 5 µIU/mL Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 5 µIU/ml - 300 µIU/mL |
| Specificity | This ELISA antibody pair detects rat, mouse, human, porcine and bovine Insulin and Proinsulin. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P01323 |
| Gene Id | 24506 |
| Gene Symbols | INS2, INS-2 |
| Protein Name / Synonyms | Insulin-1 [Cleaved into: Insulin-1 B chain Insulin-1 A chain] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
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Target Background
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Rat Insulin ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Insulin (INS), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Silicon-enriched meat consumption mitigates brain cortex damage associated with diabetic dyslipidemia in a late-stage type 2 diabetes mellitus rat model
- Species:
- Rat
- Sample Type:
- Serum (late-stage T2DM rats)
- Year:
- 2025
- Journal Name:
- Redox Biology
- PubMed ID:
- 40482347
Non-Work-Matched HIIT and MIIT Partially Restore Exerkine-Related and Mitochondrial Gene Expression in Diabetic Rat Skeletal Muscle
- Species:
- Rat
- Sample Type:
- Plasma (diabetic rats, HIIT/MIIT)
- Year:
- 2026
- Journal Name:
- Biomolecules
- PubMed ID:
- 42650818
Granular Biphasic Colloidal Hydrogels for 3D Bioprinting
- Species:
- Rat
- Sample Type:
- Other / Unspecified (insulin secreted by the encapsulated cells)
- Year:
- 2024
- Journal Name:
- Advanced Healthcare Materials
- PubMed ID:
- 38749006
Chaperone-mediated autophagy, heat shock protein 70, and serotonin: novel targets of beta-hydroxybutyrate in HFFD/LPS-induced sporadic Alzheimer's disease model
- Species:
- Rat
- Sample Type:
- Serum (HFFD/LPS rats)
- Year:
- 2025
- Journal Name:
- Inflammopharmacology
- PubMed ID:
- 40319428
Antidiabetic Effect of Substituting Processed Meat with Reduced-Fat and Diatomaceous Earth-Enriched Pâtés in Middle-Aged Female Wistar Rats
- Species:
- Rat
- Sample Type:
- Serum (middle-aged female wistar rats)
- Year:
- 2026
- Journal Name:
- Foods (Basel, Switzerland)
- PubMed ID:
- 41829151
Phyllanthus niruri niosomes ameliorate obesity-induced hepatic steatosis in rats via modulating MALAT1/miR-206/GLP-1R signaling and hepatic lipid metabolism
- Species:
- Rat
- Sample Type:
- Whole Blood (obesity-induced hepatic steatosis rats)
- Year:
- 2026
- Journal Name:
- Biological research
- PubMed ID:
- 42045980
Enhancing oral bioavailability of insulin through bilosomes: Implication of charge and chain length on apical sodium-dependent bile acid transporter (ASBT) uptake
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- International Journal of Biological Macromolecules
The potential of esculin in ameliorating Type-2 diabetes mellitus induced neuropathy in Wistar rats and probing its inhibitory mechanism of insulin aggregation
- Species:
- Rat
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- International Journal of Biological Macromolecules
Dapagliflozin mitigates cognitive deficits in a rat model of chronic restrained stress by addressing insulin resistance and mitochondrial dysfunction
- Species:
- Rat
- Sample Type:
- Serum (fasting; chronic restraint stress)
- Year:
- 2025
- Journal Name:
- Naunyn-Schmiedeberg's archives of pharmacology
- PubMed ID:
- 40397120
Evaluation of the Hepato-Pancreatic Toxicities of Selected Antibiotics in Male Wistar Rats
- Species:
- Rat
- Sample Type:
- Plasma (antibiotic-treated)
- Year:
- 2025
- Journal Name:
- Pharmacology and Toxicology of Natural Medicines (ISSN 2756-6838)