General Information
| Short Description | This OC/BGP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Rat OC/BGP protien.Detection Immunogen: Recombinant Rat OC/BGP protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein: it acts as a negative regulator of bone formation and is required to limit bone formation without impairing bone resorption or mineralization. The carboxylated form binds strongly to apatite and calcium. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. |
| Protein Name | OsteocalcinBone Gla ProteinBgpGamma-Carboxyglutamic Acid-Containing Protein |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Osteocalcin antibody pairBone Gla Protein antibody pairBgp antibody pairGamma-Carboxyglutamic Acid-Containing Protein antibody pairBglap antibody pairBglap2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat OC/BGP (Osteocalcin) Antibody Pair (STJAP00597)
This antibody pair detects rat OC/BGP (Osteocalcin), corresponding to UniProt entry P04640. Reactivity has been confirmed for rat samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated; both are mouse monoclonal antibodies provided at 0.5 mg/mL. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair has been validated for use in ELISA. It is intended for use with serum, plasma, and other biological fluid sample types.
Both the capture and detection antibodies were raised against recombinant rat OC/BGP protein. Both antibodies were purified by Protein A or G affinity chromatography.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.