Rat Slc6a4 (Sodium-dependent serotonin transporter) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	Slc6a4
Gene ID:	25553
Uniprot ID:	SC6A4_RAT
Specificity:	This kit recognizes Rat SERT in samples. No significant cross-reactivity or interference between Rat SERT and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation.
Function	Serotonin transporter that cotransports serotonin with one Na(+) ion in exchange for one K(+) ion and possibly one proton in an overall electroneutral transport cycle. Transports serotonin across the plasma membrane from the extracellular compartment to the cytosol thus limiting serotonin intercellular signaling. Essential for serotonin homeostasis in the central nervous system. In the developing somatosensory cortex, acts in glutamatergic neurons to control serotonin uptake and its trophic functions accounting for proper spatial organization of cortical neurons and elaboration of sensory circuits. In the mature cortex, acts primarily in brainstem raphe neurons to mediate serotonin uptake from the synaptic cleft back into the pre-synaptic terminal thus terminating serotonin signaling at the synapse. Modulates mucosal serotonin levels in the gastrointestinal tract through uptake and clearance of serotonin in enterocytes. Required for enteric neurogenesis and gastrointestinal reflexes. Regulates blood serotonin levels by ensuring rapid high affinity uptake of serotonin from plasma to platelets, where it is further stored in dense granules via vesicular monoamine transporters and then released upon stimulation. Mechanistically, the transport cycle starts with an outward-open conformation having Na1(+) and Cl(-) sites occupied. The binding of a second extracellular Na2(+) ion and serotonin substrate leads to structural changes to outward-occluded to inward-occluded to inward-open, where the Na2(+) ion and serotonin are released into the cytosol. Binding of intracellular K(+) ion induces conformational transitions to inward-occluded to outward-open and completes the cycle by releasing K(+) possibly together with a proton bound to Asp-98 into the extracellular compartment. Na1(+) and Cl(-) ions remain bound throughout the transport cycle. Additionally, displays serotonin-induced channel-like conductance for monovalent cations, mainly Na(+) ions. The channel activity is uncoupled from the transport cycle and may contribute to the membrane resting potential or excitability.
Protein Name	Sodium-Dependent Serotonin Transporter Sert 5ht Transporter 5htt Solute Carrier Family 6 Member 4
Database Links	Reactome: R-RNO-380615
Cellular Localisation	Cell Membrane Multi-Pass Membrane Protein Endomembrane System Endosome Membrane Synapse Cell Junction Focal Adhesion Cell Projection Neuron Projection Could Be Part Of Recycling Endosomes Density Of Transporter Molecules On The Plasma Membrane Is Itself Regulated By Stx1a Density Of Transporter Molecules On The Plasma Membrane Is Also Regulated By Serotonin Density Of Transporter Molecules Seems To Be Modulated By Itgav:itgb3
Alternative ELISA Names	Sodium-Dependent Serotonin Transporter ELISA kit Sert ELISA kit 5ht Transporter ELISA kit 5htt ELISA kit Solute Carrier Family 6 Member 4 ELISA kit Slc6a4 ELISA kit
Specificity	This kit recognizes Rat SERT in samples. No significant cross-reactivity or interference between Rat SERT and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-101	96
EDTA plasma (n=8)	89-101	94
Cell culture media (n=8)	85-100	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.56	0.90	4.46	0.55	0.93	4.16
Standard deviation	0.03	0.04	0.15	0.03	0.06	0.17
CV (%)	5.36	4.44	3.36	5.45	6.45	4.09

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance