RayBright® LIVE UV450
RayBright® LIVE UV450
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Product Description
Specifications
| Size | 100 tests, 500 tests |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | 4°C |
Description
RayBright® Live UV450 is an amine-reactive fluorescent dye that binds both live and dead cells, with a much brighter signal in dead cells than living cells which refuse the dye while dead cells are stained both on the cell surface and intracellularly. This dye can be used to discriminate live and dead cells in flow cytometry analysis and is supplied frozen in DMSO solution at a pre-titrated concentration. End users are recommended to further determine the optimal concentration for their specific cell types.
Kit Components
RayBright® Live UV450 dye is dissolved in anhydrous DMSO at pre-titrated dilution, supplied in 100 µl (100 tests) or 500 µl (500 tests).
Product Details
Conjugate/Tag/Label
RayBright® UV450
Excitation Laser
UV Laser, 305 nm
Maximum Emission Wavelength
443 nm, replace AMCA and Alexa Fluor 350 (Alexa Fluor is the trademark of Invitrogen)
Buffer
PBS, avoid protein, Tris or sodium azide.
Storage/Stability
When frozen in -20°C, it is stable for 1 year. When stored in 4°C in light-blocking condition, it is stable for 1 month.
Product Analysis
One million mouse splenocytes in 100 µl of PBS (protein free) were stained with RayBright® Live 488 at 1:200 dilution.
Note
Note: RayBright® is a trademark of RayBiotech, Inc.
