Cytometric bead array (CBA) designed to quantitatively measure 25 key inflammatory cytokines associated with cytokine storm and strongly linked to COVID-19 using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.
RayPlex® Human Cytokine Storm Bead Array (25-Plex)
RayPlex® Human Cytokine Storm Bead Array (25-Plex)
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Product Description
Specifications
| Size | 48 tests, 96 tests, 192 tests, 480 tests |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 25 |
| Solid Support | Fluorescent Microbeads |
| Design Principle | Sandwich-based |
| Method Of Detection | Flow Cytometry |
| Research Area | Inflammation, Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | Mixed storage. See datasheet. |
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Product Features
- Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| bFGF
| Eotaxin-1 (CCL11)
| GCSF
| GM-CSF
| IFN-gamma
|
| IL-10
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
| IL-2
| IL-4
| IL-5
|
| IL-6
| IL-7
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| MIP-1 alpha (CCL3)
|
| MIP-1 beta (CCL4)
| PDGF-BB
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- RayBio® Serum/Plasma Diluent
- Lyophilized Protein Standard Mix
- 1X Biotinylated Detection Antibody Cocktail
- 10X PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix (Item 5) can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
documents
citations
Akkermansia muciniphila alleviates diabetic cognitive impairment by inhibiting NLRP3 inflammasome activation and ameliorating hippocampal synaptic defects
- Year:
- 2026
- Journal Name:
- Food & Function
- PubMed ID:
- 42300325
A novel oncolytic herpes simplex virus type 2 induces polarization of tumor-associated macrophages and remodels the immune microenvironment in glioblastoma
- Year:
- 2026
- Journal Name:
- Cancer immunology, Immunotherapy
- PubMed ID:
- 42217049
Dysregulated myeloid-T cell immunity in refractory Mycoplasma pneumoniae pneumonia: high inflammation meets T cell suppression
- Species:
- Human
- Sample Type:
- Bronchoalveolar Lavage (mycoplasma pneumoniae pneumonia patients)
- Year:
- 2026
- Journal Name:
- BMC Microbiology
- PubMed ID:
- 42216148
Combined dendritic cell and anti-TIGIT immunotherapy potentiates adaptive NK cells against HIV-1
- Species:
- Human
- Sample Type:
- Other / Unspecified (not stated)
- Year:
- 2025
- Journal Name:
- EMBO Molecular Medicine
- PubMed ID:
- 40473838
Multi-omics analysis of host airway responses in pediatric Mycoplasma pneumoniae pneumonia reveals potential mechanisms of disease exacerbation caused by co-infection
- Species:
- Human
- Sample Type:
- BALF
- Year:
- 2025
- Journal Name:
- NPJ biofilms and microbiomes
- PubMed ID:
- 41413425
Novel mediator in anaphylaxis: decreased levels of miR-375-3p in serum and within extracellular vesicles of patients
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
