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> RayPlex® Human Inflammation Bead Array (6-Plex)

RayPlex® Human Inflammation Bead Array (6-Plex)

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Référence FAH-INF-2-96

Conditionnement : 96tests

Marque : RayBiotech

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RayPlex® Human Inflammation Bead Array (6,9,13-Plex)RayPlex® Human Inflammation Bead Array (6,9,13-Plex)RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

Cytometric bead array (CBA) designed to quantitatively measure 6, 9, or 13 human inflammatory factors (see panel options) using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.
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Product Description

Specifications

Size48 tests, 96 tests, 192 tests, 480 tests
SpeciesHuman
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Protein Name / Synonyms

6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha

9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta, IL-2, IL-12 p70

13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta, IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)

Quantitative/Semi-QuantitativeQuantitative
Number of Targets Detected6, 9, 13
Solid Support
Fluorescent Microbeads
Design PrincipleSandwich-based
Method Of DetectionFlow Cytometry
Research AreaInflammation
Shipping TypeBlue ice
StorageMixed storage. See datasheet.
Target Panel13-plex, 6-plex, 9-plex

Target Names

6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha

9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70

13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Application Notes

Suggested Applications
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • Lyophilized Protein Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • 10X PE Conjugate
  • Flow Cytometer Setup Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
  • RayBio® Serum/Plasma Diluent
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

documents

Datasheet (FAH-INF.pdf)
SDS (FAH-INF.pdf)
Excel (RayPlex-Analysis-Tool.xlsm)

citations

  • DNMT3B Alleviates Liver Steatosis Induced by Chronic Low-grade LPS via Inhibiting CIDEA Expression

    Species:
    Mouse
    Sample Type:
    Liver Tissue
    Year:
    2024
    Journal Name:
    Cellular and molecular gastroenterology and hepatology
    PubMed ID:
    37703946

  • Observations on the efficacy of edaravone dexborneol in preventing post-stroke depression and its inflammatory mechanism: a prospective, randomized, control trial

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2024
    Journal Name:
    Frontiers in neuroscience
    PubMed ID:
    39315079

  • Increased peripheral inflammatory responses in myelin oligodendrocyte glycoprotein associated disease and aquaporin-4 antibody positive neuromyelitis optica spectrum disorder

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Frontiers in immunology

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