RB1 siRNA (Rat)
Référence MBS829124-30nmol
Conditionnement : 30nmol
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RB1 siRNA – Retinoblastoma-Associated Protein (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829124MBS829124 is an siRNA targeting RB1. Also known as RB1 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit RB1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
RB1, siRNA
Full Product Name
RB1 siRNA (Rat)
Product Gene Name
RB1 sirna
Product Synonym Names
RB-1; Retinoblastoma-associated protein; pRb; Rb; pp105
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
105,025 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat RB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RB1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of RB1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for RB1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
retinoblastoma-associated protein
NCBI Official Synonym Full Names
retinoblastoma 1
NCBI Official Symbol
Rb1
UniProt Protein Name
Retinoblastoma-associated protein
UniProt Synonym Protein Names
pRb; Rb; pp105
Protein Family
Retinoblastoma-associated protein
UniProt Gene Name
Rb1
UniProt Synonym Gene Names
Rb-1; Rb
UniProt Entry Name
RB_RAT
NCBI Protein Information
retinoblastoma-associated protein
NCBI Summary for RB1
plays a role in negative regulation of the cell cycle and of DNA replication; acts as a tumor suppressor [RGD, Feb 2006]
UniProt Comments for RB1
Rb: retinoblastoma tumor suppressor protein regulates cell proliferation by controlling progression through the G1-phase restriction point. Has three distinct binding domains and interacts with regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif. Cell cycle-dependent phosphorylation by Cdks inhibits Rb target binding, thus allowing cell cycle progression. Recruits and targets histone methyltransferase suv39h1 leading to epigenetic transcriptional repression.
Protein type: Tumor suppressor; Oncoprotein; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Cellular Component: Rb-E2F complex; transcription factor complex; PML body; spindle; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; ubiquitin protein ligase binding; phosphoprotein binding; transcription factor binding; kinase binding
Biological Process: sister chromatid biorientation; regulation of cell cycle; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; neuron maturation; negative regulation of cell cycle; neuron differentiation; negative regulation of cell proliferation; negative regulation of mitotic cell cycle; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; chromatin modification; regulation of lipid kinase activity; enucleate erythrocyte differentiation; regulation of transcription from RNA polymerase II promoter; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; negative regulation of smoothened signaling pathway; neuron morphogenesis during differentiation; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Show more Protein type: Tumor suppressor; Oncoprotein; Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Cellular Component: Rb-E2F complex; transcription factor complex; PML body; spindle; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; ubiquitin protein ligase binding; phosphoprotein binding; transcription factor binding; kinase binding
Biological Process: sister chromatid biorientation; regulation of cell cycle; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; neuron maturation; negative regulation of cell cycle; neuron differentiation; negative regulation of cell proliferation; negative regulation of mitotic cell cycle; positive regulation of macrophage differentiation; cell cycle arrest; neurite development; negative regulation of epithelial cell proliferation; transcription, DNA-dependent; chromatin modification; regulation of lipid kinase activity; enucleate erythrocyte differentiation; regulation of transcription from RNA polymerase II promoter; myoblast differentiation; neuron apoptosis; maintenance of mitotic sister chromatid cohesion; gut development; cell division; negative regulation of smoothened signaling pathway; neuron morphogenesis during differentiation; Ras protein signal transduction; positive regulation of mitotic metaphase/anaphase transition; striated muscle cell differentiation; negative regulation of protein kinase activity; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with RB1 sirna
| Reference Product | PubMed Publications |
|---|---|
| Ccnd1 sirna | >116 publications with RB1 and Ccnd1 |
| Cdk4 sirna | >89 publications with RB1 and Cdk4 |
| Mdm2 sirna | >69 publications with RB1 and Mdm2 |
| E2f1 sirna | >57 publications with RB1 and E2f1 |
| Cdk2 sirna | >25 publications with RB1 and Cdk2 |
| Cdk6 sirna | >23 publications with RB1 and Cdk6 |
| Ccne1 sirna | >14 publications with RB1 and Ccne1 |
| E2f2 sirna | >6 publications with RB1 and E2f2 |
| Cdk1 sirna | >2 publications with RB1 and Cdk1 |
| Tfdp2 sirna | >1 publications with RB1 and Tfdp2 |
Pathways associated with RB1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| ATM Pathway antibodies | ATM Pathway Diagram |
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| Androgen Receptor Signaling Pathway antibodies | Androgen Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| Bladder Cancer Pathway antibodies | Bladder Cancer Pathway Diagram |
| Bladder Cancer Pathway antibodies | Bladder Cancer Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
Diseases associated with RB1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Retinoblastoma Antibodies | >1162 publications with RB1 and Retinoblastoma |
| Carcinoma Antibodies | >321 publications with RB1 and Carcinoma |
| Adenocarcinoma Antibodies | >141 publications with RB1 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >131 publications with RB1 and Breast Neoplasms |
| Lung Neoplasms Antibodies | >100 publications with RB1 and Lung Neoplasms |
| Neoplasms, Experimental Antibodies | >88 publications with RB1 and Neoplasms, Experimental |
| Osteosarcoma Antibodies | >77 publications with RB1 and Osteosarcoma |
| Liver Diseases Antibodies | >64 publications with RB1 and Liver Diseases |
| Necrosis Antibodies | >53 publications with RB1 and Necrosis |
| Liver Neoplasms Antibodies | >52 publications with RB1 and Liver Neoplasms |
Organs/Tissues associated with RB1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Eye Antibodies | >240 publications with RB1 and Eye |
| Brain Antibodies | >223 publications with RB1 and Brain |
| Lung Antibodies | >154 publications with RB1 and Lung |
| Liver Antibodies | >115 publications with RB1 and Liver |
| Prostate Antibodies | >65 publications with RB1 and Prostate |
| Heart Antibodies | >58 publications with RB1 and Heart |
| Colon Antibodies | >33 publications with RB1 and Colon |
| Kidney Antibodies | >31 publications with RB1 and Kidney |
| Connective Tissue Antibodies | >16 publications with RB1 and Connective Tissue |
| Ovary Antibodies | >11 publications with RB1 and Ovary |