Recombinant G Protein Coupled Receptor Kinase 6 (GRK6)
Référence MBS2029304-0,05mg
Conditionnement : 0.05mg
Marque : MyBiosource
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G Protein Coupled Receptor Kinase 6 (GRK6) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2029304SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2029304 is a recombinant protein targeting GRK6. Also known as Recombinant G Protein Coupled Receptor Kinase 6 (GRK6). Host: E.coli. More details
Product Overview
Product Name
G Protein Coupled Receptor Kinase 6 (GRK6), Recombinant Protein
Full Product Name
Recombinant G Protein Coupled Receptor Kinase 6 (GRK6)
Product Gene Name
GRK6 recombinant protein
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
FRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLF
FRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLF
Host
E.coli
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
Purity/Purification
> 95%
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: GRK6 (MBS2028800)
Immunogen: GRK6 (MBS2029304)
Immunogen: GRK6 (MBS2029304)
Matching Pairs
APC-CY7 Conjugated Antibody: GRK6 (MBS2063918)
Immunogen: GRK6 (MBS2029304)
Immunogen: GRK6 (MBS2029304)
Molecular Weight
Predicted isoelectric point: 9.1
Predicted Molecular Mass: 33.7kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Predicted Molecular Mass: 33.7kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
OMIM
600869
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Prokaryotic expression
Residues
Phe186~Phe448
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for GRK6 recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Other Notes
Small volumes of GRK6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for GRK6. It may not necessarily be applicable to this product.
NCBI Official Full Name
G protein-coupled receptor kinase 6
NCBI Official Synonym Full Names
G protein-coupled receptor kinase 6
NCBI Official Symbol
GRK6
NCBI Official Synonym Symbols
GPRK6
UniProt Protein Name
G protein-coupled receptor kinase 6
UniProt Synonym Protein Names
G protein-coupled receptor kinase GRK6
Protein Family
G protein-coupled receptor kinase
UniProt Gene Name
GRK6
UniProt Synonym Gene Names
GPRK6
UniProt Entry Name
GRK6_HUMAN
NCBI Protein Information
G protein-coupled receptor kinase 6
NCBI Summary for GRK6
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for GRK6
GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.16; AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: membrane
Molecular Function: G-protein coupled receptor kinase activity; protein binding; ATP binding
Biological Process: Wnt receptor signaling pathway; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway
Show more Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; EC 2.7.11.16; AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: membrane
Molecular Function: G-protein coupled receptor kinase activity; protein binding; ATP binding
Biological Process: Wnt receptor signaling pathway; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway
Research Articles on GRK6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with GRK6 recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| ADRB2 recombinant protein | >8 publications with GRK6 and ADRB2 |
| CXCR4 recombinant protein | >7 publications with GRK6 and CXCR4 |
| CXCL12 recombinant protein | >7 publications with GRK6 and CXCL12 |
| CXCR2 recombinant protein | >3 publications with GRK6 and CXCR2 |
| ARRB2 recombinant protein | >3 publications with GRK6 and ARRB2 |
Pathways associated with GRK6 recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| CXCR4-mediated Signaling Events Pathway antibodies | CXCR4-mediated Signaling Events Pathway Diagram |
| Calcium Regulation In The Cardiac Cell Pathway antibodies | Calcium Regulation In The Cardiac Cell Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Chemokine Signaling Pathway antibodies | Chemokine Signaling Pathway Diagram |
| Corticotropin-releasing Hormone Pathway antibodies | Corticotropin-releasing Hormone Pathway Diagram |
| Endocytosis Pathway antibodies | Endocytosis Pathway Diagram |
| Endocytosis Pathway antibodies | Endocytosis Pathway Diagram |
| FSH Signaling Pathway antibodies | FSH Signaling Pathway Diagram |
| Morphine Addiction Pathway antibodies | Morphine Addiction Pathway Diagram |
| Morphine Addiction Pathway antibodies | Morphine Addiction Pathway Diagram |
Diseases associated with GRK6 recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >21 publications with GRK6 and Nervous System Diseases |
| Disease Models, Animal Antibodies | >15 publications with GRK6 and Disease Models, Animal |
| Inflammation Antibodies | >13 publications with GRK6 and Inflammation |
| Movement Disorders Antibodies | >13 publications with GRK6 and Movement Disorders |
| Dyskinesia, Drug-Induced Antibodies | >6 publications with GRK6 and Dyskinesia, Drug-Induced |
| Mental Disorders Antibodies | >6 publications with GRK6 and Mental Disorders |
| Necrosis Antibodies | >4 publications with GRK6 and Necrosis |
| Hypertension Antibodies | >3 publications with GRK6 and Hypertension |
| Hypertrophy Antibodies | >3 publications with GRK6 and Hypertrophy |
| Poisoning Antibodies | >2 publications with GRK6 and Poisoning |
Organs/Tissues associated with GRK6 recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >30 publications with GRK6 and Brain |
| Blood Antibodies | >20 publications with GRK6 and Blood |
| Kidney Antibodies | >17 publications with GRK6 and Kidney |
| Muscle Antibodies | >16 publications with GRK6 and Muscle |
| Heart Antibodies | >11 publications with GRK6 and Heart |
| Uterus Antibodies | >7 publications with GRK6 and Uterus |
| Eye Antibodies | >6 publications with GRK6 and Eye |
| Nerve Antibodies | >6 publications with GRK6 and Nerve |
| Lung Antibodies | >5 publications with GRK6 and Lung |
| Liver Antibodies | >4 publications with GRK6 and Liver |