Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-200aa
- UniProt No.
- P26441
- NCBI Accession No.
- NP_000605
- Alternative names
- Ciliary neurotrophic factor
Product Specification
- Molecular Weight
- 25 kDa (220aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 30% glycerol, 0.2M NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1ug/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human CNTF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1ug/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.