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> Recombinant Human Prolactin Protein

Recombinant Human Prolactin Protein

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Référence 230-30003-250

Conditionnement : 250ug

Marque : RayBiotech

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Recombinant Human Prolactin ProteinRecombinant Human Prolactin Protein

Recombinant Human Prolactin Protein

Recombinant Human Prolactin Protein

Recombinant human Prolactin (PRL) protein, derived from transfected HEK293 cells
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Product Description

Specifications

Size10 µg, 50 µg, 100 µg, 250 µg
SpeciesHuman
Accession Number
P01236
Gene Id
5617
Gene Symbols
PRL
Protein Name / Synonyms
Prolactin, PRL
Expressed Region
Leu29-Cys227
Expression SystemHEK293 cells
TagN-terminal 8x histidine tag
Purity
> 95%
Purity Determined BySDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)
The predicted size of prolactin is 25 kDa. The affinity purified recombinant human prolactin showed two bands: the major band is about 25 kDa, and the minor band is about 27 kDa. Both bands were confirmed by Western blotting using anti-prolactin antibody. The 27-kDa band may represent N-glycosylated and phosphorylated form.
Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS.
Concentration (lot specific)
Determined by BCA protein assay kit (Thermo Scientific)
Preservative
None
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. Fully dissolve in freshly prepared 4 mM HCl, then add equal volume of 1x PBS (pH 7.4) to bring the concentration to 0.5 mg/mL.
Endotoxin LevelNot determined
Research AreaAngiogenesis, Cytoskeletal Reorganization
Shipping TypeAmbient temperature
Storage-20°C
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human PRL at 0.5 µg/mL (100 µL/well) can bind human PRLR (Cat. 230-30076) with EC50 at 67.5 ng/mL.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.