Recombinant Midline 2 (MID2)
Référence MBS2123501-1mg
Conditionnement : 1mg
Marque : MyBiosource
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Midline 2 (MID2) Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2123501SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2123501 is a recombinant protein targeting MID2. Also known as Recombinant Midline 2 (MID2). Host: E Coli. More details
Product Overview
Online Datasheet in PDFProduct Name
Midline 2 (MID2), Recombinant Protein
Full Product Name
Recombinant Midline 2 (MID2)
Product Gene Name
MID2 recombinant protein
Product Synonym Names
MID1; FXY2; RNF60; TRIM1; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1; Probable E3 ubiquitin-protein ligase MID2; Midline defect 2
Host
E Coli
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Purity/Purification
>95%
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Molecular Weight
Predicted Molecular Mass: 32.1kDa
Accurate Molecular Mass: 32kDa
Accurate Molecular Mass: 32kDa
Sequence Positions
Ser7~Glu258
OMIM
gene 300928
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His Tag
Subcellular Location
Secreted
Isoelectric Point
6.7
Reconstitution
Reconstitute in PBS or others.
Product Categories/Family for MID2 recombinant protein
Metabolic pathway
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for MID2 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Other Notes
Small volumes of MID2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for MID2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MID2 isoform 1
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MID2
UniProt Synonym Protein Names
Midin-2; Midline defect 2; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1
Protein Family
Cell wall integrity sensor
UniProt Gene Name
MID2
UniProt Synonym Gene Names
FXY2; RNF60; TRIM1
UniProt Entry Name
TRIM1_HUMAN
UniProt Comments for MID2
MID2: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]
Protein type: Microtubule-binding; EC 6.3.2.-; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: microtubule; cytoplasm
Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; phosphoprotein binding; ligase activity
Biological Process: negative regulation of viral transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of virion penetration into host cell; protein ubiquitination; innate immune response; positive regulation of transcription factor activity; activation of NF-kappaB transcription factor
Disease: Mental Retardation, X-linked 101
Show more Protein type: Microtubule-binding; EC 6.3.2.-; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xq22.3
Cellular Component: microtubule; cytoplasm
Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; phosphoprotein binding; ligase activity
Biological Process: negative regulation of viral transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of virion penetration into host cell; protein ubiquitination; innate immune response; positive regulation of transcription factor activity; activation of NF-kappaB transcription factor
Disease: Mental Retardation, X-linked 101
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with MID2 recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| MID1 recombinant protein | >13 publications with MID2 and MID1 |
Diseases associated with MID2 recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Metabolic Diseases Antibodies | >4 publications with MID2 and Metabolic Diseases |
| Nervous System Diseases Antibodies | >2 publications with MID2 and Nervous System Diseases |
| Lung Diseases Antibodies | >2 publications with MID2 and Lung Diseases |
| Heart Diseases Antibodies | >1 publications with MID2 and Heart Diseases |
| Urogenital Abnormalities Antibodies | >1 publications with MID2 and Urogenital Abnormalities |
| Ovarian Neoplasms Antibodies | >1 publications with MID2 and Ovarian Neoplasms |
| Mental Disorders Antibodies | >1 publications with MID2 and Mental Disorders |
| Eye Diseases Antibodies | >1 publications with MID2 and Eye Diseases |
| Fibrosis Antibodies | >1 publications with MID2 and Fibrosis |
Organs/Tissues associated with MID2 recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Embryonic Tissue Antibodies | >3 publications with MID2 and Embryonic Tissue |
| Eye Antibodies | >1 publications with MID2 and Eye |
| Lung Antibodies | >1 publications with MID2 and Lung |
| Muscle Antibodies | >1 publications with MID2 and Muscle |
| Brain Antibodies | >1 publications with MID2 and Brain |