Recombinant Rat TNFa with a C-terminal His-tag, derived from the transfected human HEK293 cells.
Recombinant Rat TNF alpha Protein
Recombinant Rat TNF alpha Protein
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Product Description
Specifications
| Size | 250 µg |
| Species | Rat |
| Accession Number | P16599 |
| Gene Symbols | TNFa |
| Protein Name / Synonyms | Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2 (TNF-a). |
| Expressed Region | G57-L235 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 22 kDa. Due to the abundant glycosylation, it migrates as approximately 26 kDa extra protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Liquid |
| Formulation | Filtered solution in 15 mM Na2HPO4 (pH 7.4), 5 mM NaH2PO4, 0.5 M NaCl, 750 mM imidazole, 20% glycerol. |
| Concentration (lot specific) | Refer to the label on the vial for the specific concentration, determined by the Pierce BCA Protein Assay Kit. |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Cardiovascular Disease, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Activity
Storage/Stability
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long-term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Generally, the shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.