Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Rhesus Monkey CD14 ELISA Kit

Rhesus Monkey CD14 ELISA Kit

Imprimer Imprimer
Identifiez-vous

Référence ELK-CD14-5

Conditionnement : 5PlateKit

Marque : RayBiotech

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Rhesus Monkey CD14 ELISA KitRhesus Monkey CD14 ELISA KitRhesus Monkey CD14 ELISA KitRhesus Monkey CD14 ELISA KitRhesus Monkey CD14 ELISA KitRhesus Monkey CD14 ELISA Kit5 Plate Kit Packaging (one box)

Rhesus Monkey CD14 ELISA Kit

Rhesus Monkey CD14 ELISA Kit

RayBio® Rhesus Monkey CD14 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman, Non-Human Primate (rhesus monkey)
Sensitivity
0.2 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.205 ng/ml- 50 ng/ml
Specificity
This ELISA antibody pair detects Rhesus Macaque CD14 and Human CD14. Other species not determined.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
100 fold
Accession Number
B3Y6B8
Gene Symbols
CD14
Protein Name / Synonyms
Monocyte differentiation antigen CD14
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCancer, Inflammation, Adaptive Immunity
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Rhesus Monkey CD14 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified CD14, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELK-CD14.pdf)
SDS (ELK-CD14.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Large expansion of plasma commensal viruses is associated with SIV pathogenesis in Macaca leonina

    Species:
    Human
    Sample Type:
    Serum (SIV-infected macaca leonina)
    Year:
    2024
    Journal Name:
    Science Advances
    PubMed ID:
    39356751

  • Alterations of the gut bacterial microbiota in rhesus macaques with SIV infection and on short- or long-term antiretroviral therapy

    Species:
    Rhesus Monkey
    Sample Type:
    Plasma
    Year:
    2020
    Journal Name:
    Scientific reports
    PubMed ID:
    33149234