lgG, H&L (AP)
Référence I1904-41Q-1mg
Conditionnement : 1mg
Marque : US Biological
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Téléphone : +1 850 650 7790
I1904-41Q lgG, H&L (AP)
Clone Type
PolyclonalHost
sheepSource
rabbitIsotype
IgGGrade
Affinity PurifiedApplications
DB E IHC WBCrossreactivity
RbShipping Temp
Blue IceStorage Temp
4°C Do Not Freeze
Applications:
Suitable for Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
ELISA: 1:1,000 to 1:5,000 Use pNPP p-nitrophenyl phosphate NPP-10 as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Applications
Product Type: Pab|Isotype: IgG|Host: sheep|Source: rabbit|Concentration: ~1mg/ml (UV280)|Form: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase (AP).|Purity: Purified by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |Immunogen: Rabbit IgG whole molecule|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-sheep serum, rabbit IgG and rabbit serum.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rabbit IgG whole molecule
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Purity
Purified by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-sheep serum, rabbit IgG and rabbit serum.