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> Sheep Decorin (DCN) ELISA Kit

Sheep Decorin (DCN) ELISA Kit

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Référence EK10858-96T

Conditionnement : 96T

Marque : Signalway Antibody

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Product Detail

Product NameSheep Decorin (DCN) ELISA Kit

ApplicationsELISA

Species ReactivitySheep (Ovis aries)

Other NamesCSCD; DSPG2; PG40; PGII; PGS2; SLRR1B; bone proteoglycan II|decorin proteoglycan|dermatan sulphate proteoglycans II|proteoglycan core protein|small leucine-rich protein 1B

Accession NoQ9TTE2

Uniprot Q9TTE2

Gene ID 443048;

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details
Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DCN in samples. An antibody specific for DCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCN bound in the initial step. The color development is stopped and the intensity of the color is measured.

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