Sheep Interleukin 1 Beta (IL1b) ELISA Kit

Référence MBS2025006-48

Conditionnement : 48-Strip-Wells

Marque : MyBiosource

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Interleukin 1 Beta (IL1b) ELISA Kit for Sheep

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025006
SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter… More details

Product Overview

Online Datasheet in PDF
Product Name
Interleukin 1 Beta (IL1b), ELISA Kit
Full Product Name
Sheep Interleukin 1 Beta (IL1b) ELISA Kit
Product Gene Name
IL1b elisa kit
Product Synonym Names
IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin; IL1-B; IL1-Beta; IL1F2; IL-1b; Interleukin-1 Family Member 2; Catabolin
Species Reactivity
Sheep
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
7.8-500pg/mL
Sensitivity
2.8pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%

Product Categories/Family for IL1b elisa kit

Cytokine; Tumor immunity; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of IL1b elisa kit

Sheep IL1b ELISA Kit – Cat# MBS2025006

Other Notes

Small volumes of IL1b elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL1b purchase

MBS2025006 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1 Beta (IL1b) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL1b. The ELISA analytical biochemical technique of the MBS2025006 kit is based on IL1b antibody-IL1b antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1b antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1b. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL1b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
interleukin 1, beta
NCBI Official Symbol
IL1B2
NCBI Official Synonym Symbols
IL1B; IL-1beta
Protein Family
Interleukin
NCBI Protein Information
interleukin 1, beta

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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