Sheep PAG1(Pregnancy associated glycoprotein 1) ELISA Kit

Référence orb1146844-96

Conditionnement : 96Test

Marque : Biorbyt

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Sheep PAG1(Pregnancy associated glycoprotein 1) ELISA Kit

Catalog Number: orb1146844

Catalog Numberorb1146844
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy associated glycoprotein 1(PAG1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnancy associated glycoprotein 1(PAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy associated glycoprotein 1(PAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy associated glycoprotein 1(PAG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivitySheep
Range0.16-10 ng/mL
UniProt IDQ28755
Sensitivity0.055 ng/mL
Protein Length3.5h
Alternative namesPAG1, Pregnancy associated glycoprotein 1
NoteFor research use only
Sample Typesserum, plasma and other biological fluids
Expiration DatePlease enquire.
Sheep PAG1(Pregnancy associated glycoprotein 1) ELISA Kit