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> Sheep Prolactin-releasing peptide (PRLH) ELISA Kit

Sheep Prolactin-releasing peptide (PRLH) ELISA Kit

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Référence EK8225-96T

Conditionnement : 96T

Marque : Signalway Antibody

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Product Detail

Product NameSheep Prolactin-releasing peptide (PRLH) ELISA Kit

ApplicationsELISA

Species ReactivitySheep (Ovis aries)

Other NamesPRH; PRRP; preproprolactin-releasing peptide

Accession NoQ8WN12

Uniprot Q8WN12

Gene ID 443466;

StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

Application Details
Detect Range:Request Information
Sensitivity:Request Information
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm
Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PRLH in samples. An antibody specific for PRLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue.

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