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> Sheep Rabbit IgM (Fc specific), conjugated with TRITC Antibody

Sheep Rabbit IgM (Fc specific), conjugated with TRITC Antibody

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Référence orb21363-1ml

Conditionnement : 1ml

Marque : Biorbyt

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Sheep Rabbit IgM (Fc specific), conjugated with TRITC Antibody

SKU: orb21363
This product can be custom made

Description

Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal sheep antiserum to Rabbit IgM, Fc specific. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used.

Research Area

Stem Cells

Images & Validation

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Sheep Rabbit IgM (Fc specific), conjugated with TRITC Antibody
ReactivityRabbit

Key Properties

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Antibody TypeSecondary Antibody
HostSheep
ClonalityPolyclonal
ConjugationTRITC

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Form/AppearanceTRITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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