Disease:

Ovarian serous cystadenocarcinoma

Origin:

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumor. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Species:

Homo sapiens, human

Tissue:

Ovary: ascites

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Hypodiploid to hypotetraploid

Patient:

Female, Caucasian, 64 years of age

Medium:

AddexBio-Formulated DMEM (C0003-03) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:2 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37°C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO