Biovalley > Spaca6 (NM_001162909) Mouse Untagged Clone
Spaca6 (NM_001162909) Mouse Untagged Clone
Référence MC211601
Conditionnement : 10ug
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Spaca6 (NM_001162909) Mouse Untagged Clone
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SKU
MC211601
Ncrna00085 (untagged) - Mouse non-protein coding RNA 85 (Ncrna00085), (10ug)
ORF Plasmid:
Tag Free tGFP DDK
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Spaca6 |
| Synonyms | 4930546H06Rik; 9530079D04Rik; B230206P06Rik; let-7e; mir-99b; mir-125a; Ncrna00085 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MC211601 representing NM_001162909 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCTCACAAAGGTCACTATCTTCTCCTCAGACCCGGAGACCCTCTGTTATGGGTCTTATTTCCTTAG TAGGGAGCATTGTCCTGTTGTTCCTGCTGATCTTCAGGGCATCCACGTGGGCCTGTCTCTTCTGCTTCAC TACCTATGAGGAACGCCTCCGTGTCTGCCAGCTGTTTGTGGGCAGAGAGGAAACCAAGATAAACCTGTGC AGAAATGAGCTTGAAGGGGCTTTTGAAGATCTCAAAGACATGAAAATCAACTATGATGAGAGGAGCTACC TGCATGATGAATTTACACAGATGACAGTTTCTCTTCAGGAGAAGGCTGCTAGACGACGGGAGCCCTTTTG GCTTGCCTTCAAAGATGCTGCTGCAAAATTGAAGAGGACCATTGAACACCTTAAAAAAGCCCCGGCCTGC ATCCCTCCTTGTGGTCTCCAGGAGGTGGCTAGGCTTTTCCACTGCTCGGGCTGCTTTTCTAAACTCTGCG ATCTGCCTCTCGATTGTCCAGTTCAGGACATGCTGGTGAACCGAGGGGACCAGGCTTTGTTTTCTTGTAT CGTGGCTTTCGAGTTGCCAGAGTCGGAGATCACTTATTCCTGGAAATTTGTGGGAGGTGTCCGAACTAAG GACGTTACGTACTTCCGTGATATGCCGGGGGCTCACGGGTACCTGGCACGAATACGGCCAGTGCAACCCA AACACGGTGGGACCTTTTCCTGCGTGATCCTGCACGACCAGCGCCCCCTGGCGCGGCTCTACTTTTATCT GAACGTGACGGGCCCTCCTCCGCCTGAGGACACTGAGCTCCAGGTCACGTTCCGGGAAGTGATGAATCGG ACGCCGGCGGAGCCGGAAATGATCCAGCCCTGGAGCCCCAGCCTAGGCGAACTACTTACCAACCCCCAGG CTCTGACGCTTGGCAACCTATTCCTGCTCGCGGCCACCGCTGCACTTGGGTCTGCCAGTGTTACTCTGCT AGTGTGGCTGTTTTTTCGATGGTACTTAAGTGGCAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_001162909 |
| Insert Size | 1020 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biovalley.fr or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001162909.1, NP_001156381.1 |
| RefSeq Size | 1102 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 75202 |
| UniProt ID | E9Q8Q8 |
| Cytogenetics | 17 A3.2 |
| Summary | Sperm protein potentially involved sperm-egg fusion.UniProtKB/Swiss-Prot Function |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| MG224526 | Ncrna00085 (tGFP-tagged) - Mouse non-protein coding RNA 85 (Ncrna00085), (10ug) | 10 ug | | |
| MR224526 | Ncrna00085 (Myc-DDK-tagged) - Mouse non-protein coding RNA 85 (Ncrna00085) | 10 ug | | |
| MR224526L3 | Lenti ORF clone of Ncrna00085 (Myc-DDK-tagged) - Mouse non-protein coding RNA 85 (Ncrna00085) | 10 ug | | |
| MR224526L4 | Lenti ORF clone of Ncrna00085 (mGFP-tagged) - Mouse non-protein coding RNA 85 (Ncrna00085) | 10 ug | | |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.