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> Squalene [111-02-4]

Squalene [111-02-4]

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Référence HY-N1214-1mL

Conditionnement : 10mM/1mL

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Description

Squalene (Super Squalene) is an intermediate product in the synthesis of cholesterol, and shows several pharmacological properties such as hypolipidemic, hepatoprotective, antiatherosclerotic, cardioprotective, antioxidant, and antitumour activity[1][2].

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

Cellular Effect
Cell Line Type Value Description References
MCF7 IC50
>50 μM
Compound: 20
Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
NCI-H460 IC50
>50 μM
Compound: 20
Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human NCI-H460 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
Neutrophil IC50
>30 μM
Compound: 8
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced superoxide anion generation treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge by spectrophotometry
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced superoxide anion generation treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge by spectrophotometry
[PMID: 19203247]
Neutrophil IC50
>30 μM
Compound: 8
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
Antiinflammatory activity in human neutrophils assessed as inhibition of fMet-Leu-Phe/Cytochalasin B-induced elastase release treated 5 mins before fMet-Leu-Phe/Cytochalasin B challenge
[PMID: 19203247]
SF-268 IC50
>50 μM
Compound: 20
Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay
Cytotoxicity against human SF268 cells after 72 hrs by MTS reduction assay
[PMID: 20704331]
In Vitro

Squalene (12.5, 50 and 200 μM; 24 h) effects on MCF10A epithelial cells in a dose-dependent manner: (a) it decreases intracellular ROS level, (b) it prevents H2O2-induced oxidative injury, and (c) it protects against oxidative DNA damage[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Squalene Related Antibodies
In Vivo

Squalene (0.25-1 g/kg; fed chow; diets for 11 weeks) promotes changes in HDL- cholesterol and paraoxonase 1 and decreases reactive oxygen species in lipoproteins and plasma malondialdehyde levels[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male mouse models (wild-type, Apoa1- and Apoe- deficient)[3]
Dosage: 0.25 g/kg, 1 g/kg
Administration: Fed chow; diets for 11 weeks
Result: Increased high density lipoprotein-cholesterol and paraoxonase 1 and decreases oxidative stress in mice.
Essai clinique
Masse moléculaire

410.72

Formule

C30H50
CAS No.
Appearance

Liquid (Density: 0.858 g/cm3)

Color

Colorless to light yellow

SMILES

C/C(C)=C\CC/C(C)=C/CC/C(C)=C/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)\C
Structure Classification
Initial Source

Widespread
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

Ethanol : 20 mg/mL (48.69 mM; ultrasonic and warming and heat to 60°C)

DMSO : 16.67 mg/mL (40.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4347 mL 12.1737 mL 24.3475 mL
5 mM 0.4869 mL 2.4347 mL 4.8695 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (4.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.67 mg/mL (4.07 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Pureté et documentation
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