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> SR Proteins (Non-snRNP Splicing Factor) (APC)

SR Proteins (Non-snRNP Splicing Factor) (APC)

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Référence S6570-50-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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S6570-50-APCSR Proteins (Non-snRNP Splicing Factor) (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
SourceXenopus
IsotypeIgG1,k
GradeAffinity Purified
ApplicationsFLISA IF WB
CrossreactivityHu Xe
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

SR proteins are family of highly conserved arginine/serine rich, spliceosome associated phosphoproteins essential for metazoan pre-mRNA splicing (3,4). SR proteins appear to act early in splicing by promoting splice site recognition and spliceosome assembly. SR proteins also play a regulatory role, because they can determine alternative splice site usage in vivo and in vitro (5-7); a particularly interesting example being the regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins (8). SR proteins appear to be recruited from nuclear “speckles,” in which they are concentrated, to sites of transcription in order to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus (1,9).

Applications:
Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 3G270|Host: mouse|Source: Xenopus|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: Mass-isolated nuclei from Xenopus Laevis oocytes.|Specificity: Recognizes Xenopus SR proteins. Reacts with all members of the SR protein family. The epitope is phosphorylation-dependent so care must be taken to avoid dephosphorylation. Species Crossreactivity: human||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mass-isolated nuclei from Xenopus Laevis oocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Xenopus SR proteins. Reacts with all members of the SR protein family. The epitope is phosphorylation-dependent so care must be taken to avoid dephosphorylation. Species Crossreactivity: human
References
1. Tuma, R, et al. J. Cell Biol. 122: 767-773 (1993). 2. Neugebauer, KM and Roth, MB Genes and Development 11: 1148-1159 (1997). 3. Tacke R and Manley JL, Proc Soc Exp Biol Med 220: 59-63 (1999). 4. Graveley BR et al., Curr Biol 9: R6-7 (1999). 5. Fu X-D, Nature 365: 82-85 (1993). 6. Zahler AM, et al., Science 260: 219-222 (1993). 7. Caceres JF, Science 265: 1706-1709 (1994). 8. Kanopka A et al., Nature 393: 185-7 (1998). 9. Misteli T et al., Nature 387: 523-527 (1997).