TAT siRNA (Mouse)
Référence MBS827827-30nmol
Conditionnement : 30nmol
Marque : MyBiosource
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TAT siRNA – Tyrosine Aminotransferase (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827827MBS827827 is an siRNA targeting TAT. Also known as TAT siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit TAT expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TAT, siRNA
Full Product Name
TAT siRNA (Mouse)
Product Gene Name
TAT sirna
Product Synonym Names
Tyrosine aminotransferase; TAT; L-tyrosine:2-oxoglutarate aminotransferase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
50,565 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TAT sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TAT sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TAT. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tyrosine aminotransferase
NCBI Official Synonym Full Names
tyrosine aminotransferase
NCBI Official Symbol
Tat
UniProt Protein Name
Tyrosine aminotransferase
UniProt Synonym Protein Names
L-tyrosine:2-oxoglutarate aminotransferase
Protein Family
Protein
UniProt Gene Name
Tat
UniProt Synonym Gene Names
TAT
UniProt Entry Name
ATTY_MOUSE
NCBI Protein Information
tyrosine aminotransferase
NCBI Summary for TAT
This gene encodes a liver-specific mitochondrial enzyme that catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Regulated by glucocorticoid and polypeptide hormones, this gene's expression is affected by deletion of a regulatory region near the albino locus on chromosome 7. Mutations in this gene cause tyrosinemia type II in humans. [provided by RefSeq, Mar 2010]
Show more UniProt Comments for TAT
TAT: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine. Defects in TAT are the cause of tyrosinemia type 2 (TYRO2); also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Transferase; EC 2.6.1.5; Mitochondrial; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Amino Acid Metabolism - tyrosine
Cellular Component: mitochondrion
Molecular Function: transferase activity; amino acid binding; tyrosine transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding
Biological Process: response to mercury ion; amino acid metabolic process; glutamate metabolic process; L-phenylalanine catabolic process; tyrosine catabolic process; aromatic amino acid family metabolic process; response to glucocorticoid stimulus; biosynthetic process; aromatic amino acid family catabolic process; response to oxidative stress; response to organic cyclic substance; 2-oxoglutarate metabolic process
Show more Protein type: Transferase; EC 2.6.1.5; Mitochondrial; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - cysteine and methionine; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Amino Acid Metabolism - tyrosine
Cellular Component: mitochondrion
Molecular Function: transferase activity; amino acid binding; tyrosine transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding
Biological Process: response to mercury ion; amino acid metabolic process; glutamate metabolic process; L-phenylalanine catabolic process; tyrosine catabolic process; aromatic amino acid family metabolic process; response to glucocorticoid stimulus; biosynthetic process; aromatic amino acid family catabolic process; response to oxidative stress; response to organic cyclic substance; 2-oxoglutarate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TAT sirna
| Reference Product | PubMed Publications |
|---|---|
| Tyr sirna | >98 publications with TAT and Tyr |
| Pah sirna | >34 publications with TAT and Pah |
| Ddc sirna | >10 publications with TAT and Ddc |
| Prdx6 sirna | >2 publications with TAT and Prdx6 |
| Hpd sirna | >1 publications with TAT and Hpd |
Pathways associated with TAT sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Amino Acid Metabolism Pathway antibodies | Amino Acid Metabolism Pathway Diagram |
| Biosynthesis Of Amino Acids Pathway antibodies | Biosynthesis Of Amino Acids Pathway Diagram |
| Biosynthesis Of Amino Acids Pathway antibodies | Biosynthesis Of Amino Acids Pathway Diagram |
| Biosynthesis Of Antibiotics Pathway antibodies | Biosynthesis Of Antibiotics Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Metabolism Of Amino Acids And Derivatives Pathway antibodies | Metabolism Of Amino Acids And Derivatives Pathway Diagram |
| Methionine Salvage Pathway antibodies | Methionine Salvage Pathway Diagram |
Diseases associated with TAT sirna
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >1012 publications with TAT and Cardiovascular Diseases |
| Nervous System Diseases Antibodies | >686 publications with TAT and Nervous System Diseases |
| Brain Diseases Antibodies | >559 publications with TAT and Brain Diseases |
| Inflammation Antibodies | >509 publications with TAT and Inflammation |
| Necrosis Antibodies | >411 publications with TAT and Necrosis |
| Heart Diseases Antibodies | >360 publications with TAT and Heart Diseases |
| Liver Diseases Antibodies | >249 publications with TAT and Liver Diseases |
| Neoplasms, Experimental Antibodies | >185 publications with TAT and Neoplasms, Experimental |
| Liver Neoplasms Antibodies | >179 publications with TAT and Liver Neoplasms |
| Kidney Diseases Antibodies | >144 publications with TAT and Kidney Diseases |
Organs/Tissues associated with TAT sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Liver Antibodies | >692 publications with TAT and Liver |
| Muscle Antibodies | >305 publications with TAT and Muscle |
| Lung Antibodies | >267 publications with TAT and Lung |
| Embryonic Tissue Antibodies | >94 publications with TAT and Embryonic Tissue |
| Pancreas Antibodies | >59 publications with TAT and Pancreas |
| Stomach Antibodies | >33 publications with TAT and Stomach |
| Uncharacterized Tissue Antibodies | >1 publications with TAT and Uncharacterized Tissue |