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> TEV Protease Protein, TEV, Recombinant (His)

TEV Protease Protein, TEV, Recombinant (His)

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Référence NB-64-128237-1000U

Conditionnement : 1000U

Marque : Neo Biotech

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TEV Protease Protein, TEV, Recombinant (His)

(Synonyms: TEV, rTEV, P1 protease) Copy Product Info

Synonyms: TEV, rTEV, P1 protease

Catalog No. TMPS-00131 Copy Product Info
Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.

The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography.

The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% by SDS-PAGE analyses.
Color:Transparent
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Product Introduction

Bioactivity
Bioactivity
10 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.
Description
Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.

The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates. TEV protease includes an N-terminal 6×His tag, allowing for convenient removal after digestion via Ni²⁺ affinity chromatography.

The recombinant enzyme consists of 231 amino acids, has a molecular mass of approximately 28.4 kDa, and is fully biologically active following purification.
TagHis
Protein Purity
> 95% as determined by SDS-PAGE
FormulationSterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5.
SynonymsTEV, rTEV, P1 protease
Research Background
Tobacco Etch Virus (TEV) protease is a highly site-specific cysteine protease commonly used to remove affinity tags from fusion proteins. It recognizes the sequence Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser (ENLYFQ↓G/S), with cleavage occurring between the Gln and Gly or Ser residue. The most frequently used recognition site is ENLYFQG.

The optimal cleavage temperature is 30 °C, though conditions such as enzyme amount, incubation time, and temperature may require optimization for different substrates.
Chemical Properties
Molecular Weight28.4 kDa (Reducing conditions)
Storage & Solubility Information
ShippingProteins are shipped with blue ice.
StorageRecombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

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Related Tags: TEV Protease Protein, TEV, Recombinant (His) chemical structure | TEV Protease Protein, TEV, Recombinant (His) molecular weight