Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis^[1].

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of MTT solution
Dissolve MTT in anhydrous DMSO to obtain a 5 mg/mL solution.
2. Cell proliferation assay (96-well plate)
2.1 Cell inoculation: Prepare single cells and prepare a suspension with 10% FBS culture medium. Inoculate them into a 96-well plate, with 1000-10,000 cells per well, and each well volume is 100 μL.
2.2 Cell culture: At 37°C, with 5% CO2, incubate for 24-72 hours.
2.3 Add 10 μL of MTT to each well, incubate for 4 hours, discard the supernatant, and the cells need to be centrifuged first.
2.4 Add 100 μL of DMSO, shake for 10 minutes to completely dissolve the crystals.
2.5 Use an absorbance plate reader to monitor the increase in absorbance at OD = 562 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.  [Content Brief]

  [2]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.  [Content Brief]