Thyroid Hormone Receptor Antibody, Clone H43: ATTO 488
Référence MBS8005759-0,1mg
Conditionnement : 0.1mg
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Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43 (SMC-561). Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody (SMC-561) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.
Thyroid Hormone Receptor (THRA) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8005759Background: Thryoid hormone receptors are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. Thyroid hormones affect metabolic processes, growth and development. More details
Product Overview
Online Datasheet in PDFProduct Name
Thyroid Hormone Receptor (THRA), Monoclonal Antibody
Full Product Name
Thyroid Hormone Receptor Antibody, Clone H43: ATTO 488
Product Gene Name
anti-THRA antibody
Product Synonym Names
Mouse Anti-Human Thyroid Hormone Receptor Monoclonal IgG2a; ERBA ALPHA antibody; ERBA beta antibody; ERBA1 antibody; ERBA2 antibody; NR1A1 antibody; NR1A2 antibody; THR1 antibody; THRA antibody THRA1 antibody; THRA2 antibody; THRB antibody; THRB1 antibody; THRB2 antibody; Thyroid hormone receptor alpha antibody; Thyroid hormone receptor beta antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
H43
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Purity/Purification
Protein A Purified
Concentration
1mg/ml (lot specific)
Specificity
Detects 70 kDa.
Preparation and Storage
Store at -20 degree C
Shipping Temperature: Blue Ice or 4 degree C
Shipping Temperature: Blue Ice or 4 degree C
OMIM
190120
Immunogen
Synthetic peptide from the full length Human Thyroid hormone receptor protein
Immunogen Species
Human
Tissue Specificity
Activated T lymphocytes
Conjugate
ATTO 488
Cellular Localization
Nuclear
Certificate of Analysis
A 1:500 dilution was sufficient for detection of Pan-Thyroid hormone receptor in 10 ug of Hep G2 Human Hepatoblastoma Cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Product Categories/Family for anti-THRA antibody
Cell Signaling; Epigenetics and Nuclear Signaling; Growth Factors; Neuroscience; Nuclear Signaling Pathways; Antibodies
Applications Tested/Suitable for anti-THRA antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-THRA antibody
WB: 1:500
Western Blot (WB) of anti-THRA antibody
Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43 (SMC-561). Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody (SMC-561) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band(s): Higher molceular weight bands could be due to PTMs.
Other Notes
Small volumes of anti-THRA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for THRA purchase
MBS8005759 is an antibody targeting THRA. Also known as Thyroid Hormone Receptor Antibody, Clone H43: ATTO 488. Reactive in Human, Mouse, Rat. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for THRA. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
thyroid hormone receptor alpha isoform 3
NCBI Official Synonym Full Names
thyroid hormone receptor alpha
NCBI Official Symbol
THRA
NCBI Official Synonym Symbols
AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
UniProt Protein Name
Thyroid hormone receptor alpha
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha
Protein Family
Bifunctional aspartokinase/homoserine dehydrogenase
UniProt Gene Name
THRA
UniProt Synonym Gene Names
EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
UniProt Entry Name
THA_HUMAN
NCBI Protein Information
thyroid hormone receptor alpha
NCBI Summary for THRA
The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for THRA
TR-alpha: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Interacts with SRC-3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Four splice variant isoforms have been described.
Protein type: DNA-binding; Transcription factor; Nuclear receptor
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; nucleus; cytosol
Molecular Function: protein domain specific binding; protein binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation
Disease: Hypothyroidism, Congenital, Nongoitrous, 6
Show more Protein type: DNA-binding; Transcription factor; Nuclear receptor
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; nucleus; cytosol
Molecular Function: protein domain specific binding; protein binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; TATA-binding protein binding; protein complex binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of transcriptional preinitiation complex assembly; cytoplasmic sequestering of transcription factor; ossification; intracellular receptor-mediated signaling pathway; hormone-mediated signaling; positive regulation of female receptivity; regulation of myeloid cell apoptosis; regulation of heart contraction; regulation of lipid catabolic process; regulation of transcription from RNA polymerase II promoter; learning and/or memory; female courtship behavior; thyroid gland development; erythrocyte differentiation; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to cold; negative regulation of transcription, DNA-dependent; cartilage condensation
Disease: Hypothyroidism, Congenital, Nongoitrous, 6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-THRA antibody
| Reference Product | PubMed Publications |
|---|---|
| CCND1 antibody | >3 publications with THRA and CCND1 |
| MED1 antibody | >2 publications with THRA and MED1 |
| RXRG antibody | >1 publications with THRA and RXRG |
| RXRB antibody | >1 publications with THRA and RXRB |
| RXRA antibody | >1 publications with THRA and RXRA |
| NCOR1 antibody | >1 publications with THRA and NCOR1 |
| ESR1 antibody | >1 publications with THRA and ESR1 |
| CREBBP antibody | >1 publications with THRA and CREBBP |
Pathways associated with anti-THRA antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Endochondral Ossification Pathway antibodies | Endochondral Ossification Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Generic Transcription Pathway antibodies | Generic Transcription Pathway Diagram |
| Neuroactive Ligand-receptor Interaction Pathway antibodies | Neuroactive Ligand-receptor Interaction Pathway Diagram |
| Neuroactive Ligand-receptor Interaction Pathway antibodies | Neuroactive Ligand-receptor Interaction Pathway Diagram |
| Nuclear Receptor Transcription Pathway antibodies | Nuclear Receptor Transcription Pathway Diagram |
| Nuclear Receptors Pathway antibodies | Nuclear Receptors Pathway Diagram |
Diseases associated with anti-THRA antibody
| Disease Name | Pubmed Publications |
|---|---|
| Cardiovascular Diseases Antibodies | >17 publications with THRA and Cardiovascular Diseases |
| Thyroid Diseases Antibodies | >12 publications with THRA and Thyroid Diseases |
| Nervous System Diseases Antibodies | >4 publications with THRA and Nervous System Diseases |
| Brain Diseases Antibodies | >3 publications with THRA and Brain Diseases |
| Liver Diseases Antibodies | >3 publications with THRA and Liver Diseases |
| Kidney Diseases Antibodies | >2 publications with THRA and Kidney Diseases |
| Drug Toxicity Antibodies | >2 publications with THRA and Drug Toxicity |
| Weight Loss Antibodies | >2 publications with THRA and Weight Loss |
| Heart Diseases Antibodies | >2 publications with THRA and Heart Diseases |
| Fatty Liver Antibodies | >2 publications with THRA and Fatty Liver |
Organs/Tissues associated with anti-THRA antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Thyroid Antibodies | >43 publications with THRA and Thyroid |
| Blood Antibodies | >21 publications with THRA and Blood |
| Bone Antibodies | >14 publications with THRA and Bone |
| Brain Antibodies | >11 publications with THRA and Brain |
| Liver Antibodies | >9 publications with THRA and Liver |
| Muscle Antibodies | >5 publications with THRA and Muscle |
| Ear Antibodies | >4 publications with THRA and Ear |
| Heart Antibodies | >4 publications with THRA and Heart |
| Testis Antibodies | >3 publications with THRA and Testis |
| Eye Antibodies | >3 publications with THRA and Eye |