Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Tissue cDNA, First Strand, Mouse Adult Normal, Heart, BioGenomics™

Tissue cDNA, First Strand, Mouse Adult Normal, Heart, BioGenomics™

Imprimer Imprimer
Identifiez-vous

Référence T5595-0667-40T

Conditionnement : 40Tests

Marque : US Biological

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

T5595-0667Tissue cDNA, First Strand, Mouse Adult Normal, Heart, BioGenomics™

Specifications
GradeMolecular Biology Grade
Shipping TempDry Ice
Storage Temp-20°C

BioGenomics™ Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.

PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA. It can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from documented human adult normal tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume.
RT Reaction stopped by heating at 65°C for 10 minutes.

Features:
• Ready to use for PCR
• Oligo dT primer used to ensure the entire 3' end of cDNA is present
• With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA.

Applications:
• Immediate PCR Amplification of known genes.
• Verification of genetic mutation
• Comparison of a specific gene between different tissues.
• Analysis of mRNA alternative splicing Gene cloning and target sequencing.

Quality Control
1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectro- photometer. A260/280 is between 1.8-2.0 (detected in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥ 1.
2. The RNA used for cDNA synthesis is treated by DNase I. It is tested as DNA free RNA by PCR.
3. The synthesized cDNA was used as template for PCR amplification of b-actin gene and clathrin gene (6k region). A 838 bp b-actin band and a 565 bp clathrin band were visualized on 1% agarose gel.
4. b-actin control primer pair is included in each package. It is enough for 10 PCR reactions.

Storage : Store cDNAs at -20°C

Applications
Concentration: ~2.5-5.0ng/ul||Form: Supplied as a liquid in 1X RT buffer. (1X RT Buffer: 50 mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCI2, 10mM DTT).||Unit: 1ul cDNA is good for one PCR reaction.||Donor Informatin: Lot-to-lot specific||Supplied with: T5595-0667A: Tissue, cDNA, First Strand, Mouse Adult Normal, Heart, Actin Control Primer||Control PCR condition:|PCR Ready First Strand cDNA 1ul|10 x PCR Buffer 2.5ul|2.5 mM dNTP 4ul|Control primers (25uM) 1ul|H2O, Nuclease-free 16.3ul|Taq Polymerase(5u/ul) 0.2ul|The PCR thermocycling: |94°C x 2 minutes|94°C x 30 seconds, 55°C x 45 seconds, 72°C x 30 seconds, 35 cycles 72°C x 5 minutes. Then hold at 4°C.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.