Tissue, Total RNA, Rat Normal, Embryo (17 days), BioGenomics
Référence MBS638767-0,05mg
Conditionnement : 0.05mg
Marque : MyBiosource
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Téléphone : +1 850 650 7790
Tissue, Total RNA, Rat Normal, Embryo RNA Extraction
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638767Total RNAs are available from almost 200 different human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection,… More details
Product Overview
Online Datasheet in PDFProduct Name
Tissue, Total RNA, Rat Normal, Embryo, RNA Extraction
Full Product Name
Tissue, Total RNA, Rat Normal, Embryo (17 days), BioGenomics
Form/Format
Supplied as a liquid in RNA Storage Buffer: 0.1% DEPC-treated water with 0.1mM EDTA. No non-specific endonucleases, no nickase, no exonuclease and no RNase detected.
Purity/Purification
Molecular Biology Grade
Total RNA isolated by modified guanidine thiocyanate techniques.
Total RNA isolated by modified guanidine thiocyanate techniques.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -70 degree C in RNA storage solution. Aliquots are stable for at least 6 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Features
Total RNA isolated from a wide variety of hard to obtain tissues
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Available documentation of tissues' clinical histories (additional cost)
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Available documentation of tissues' clinical histories (additional cost)
Quality Control
1. The integrity of the RNA is examined by visual inspectiuon for the presence of intact bands of 18s and 28s RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotomer. (A260/280 = 1.8-2.0 detected in 10mM Tris-Cl, pH 7.5).
2. The RNA is treated by DNAse I. Iis tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
2. The RNA is treated by DNAse I. Iis tested as DNA free RNA by PCR.
3. cDNA synthesis is successfully performed by using this RNA as template.
Product Categories/Family for Tissue, Total RNA, Rat Normal, Embryo rna extraction
Cells; Lysates; Plasma; Serum; Tissues; Tissues-Total RNA
Application Notes for Tissue, Total RNA, Rat Normal, Embryo rna extraction
Northern Blotting, RT-PCR, and RACE, cDNA synthesis and cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection and primer extension, RNA display, Purification of mRNA
Other Notes
Small volumes of Tissue, Total RNA, Rat Normal, Embryo rna extraction vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Tissue, Total RNA, Rat Normal, Embryo purchase
MBS638767 is an RNA extraction targeting Tissue, Total RNA, Rat Normal, Embryo. Also known as Tissue, Total RNA, Rat Normal, Embryo (17 days), BioGenomics.