| Size | |
|---|---|
| 100µg | Inquiry |
Dispatch Time: About 3 working days
- Product Name
- TJP1 antibody
- Catalogue No.
- FNab09751
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tight junction protein 1(zona occludens 1)
- Alternative Names
- Tight junction protein ZO-1|Tight junction protein 1|Zona occludens protein 1|Zonula occludens protein 1|TJP1|ZO1 antibody
- UniProt ID
- Q07157
- Observed MW
- 230 kDa
Application
- Tested Applications
- ELISA, WB, IHC, FC, IF, IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09751(ZO1 antibody) at dilution of 1:500 
IP result of anti-ZO-1(IP:FNab09751, 4ug; Detection:FNab09751 1:500) with HEK-293 cells lysate 2320 ug 
Immunohistochemistry of paraffin-embedded human kidney using FNab09751(ZO1 antibody) at dilution of 1:100 
Immunofluorescent analysis of MCF7 cells using FNab09751(ZO-1 Antibody) at dilution of 1:100 - Background
- Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).