TMB ELISA Substrate
Référence MBS539294-5x100mL
Conditionnement : 5x100mL
Marque : MyBiosource
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TMB Substrate
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS539294MBS539294 is a substrate targeting TMB. Also known as TMB ELISA Substrate. High sensitivity HRP microwell substrate (containing TMB) More details
Product Overview
Online Datasheet in PDFProduct Name
TMB, Substrate
Full Product Name
TMB ELISA Substrate
Product Gene Name
TMB substrate
Form/Format
Liquid ultra sensitive substate for ELISA mircowell applications containing TMB (3,3,5,5-tetramethylbenzidine) in a mildly acidic buffer. Mixed substrate/chromogen. Excellent for microwell peroxidase immunoassay systems
Concentration
Ready to use @ 100ul per well
Use this product at room temperature (lot specific)
Use this product at room temperature (lot specific)
Preparation and Storage
Store at 2-8 degree C. Product is light sensitive: protect from direct sunlight and UV sources.
Biohazard
Non-hazardous, mild oxidizing agent, no organic solvents. Use good laboratory practices when handling; in case of contact, flush with water.
pH
3.55
Product Categories/Family for TMB substrate
Biological Reagents; Substrates
Applications Tested/Suitable for TMB substrate
ELISA (EIA)
Application Notes for TMB substrate
In kinetic assays, TMB developes a deep blue color in the presence of peroxidase and can be read at 370 or 650nm. In endpoint assays, TMB develops a yellow color and can be read at 450nm approximately 30 to 60 minutes after adding stopping reagent.
Other Notes
Small volumes of TMB substrate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.