TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450 nm. This substrate gives higher signal and has a higher sensitivity in ELISA than OPD (ortho-phenylenediamine dichloride).

TMB ONE characteristics are:

• “The Gold Standard” used across the industry
• Incredibly stable and robust
• Aqueous based – no organic solvents (REACH Annex XVII compliant)
• Low lot-to-lot variation
• Suitable for use in electrochemical assays

For traceable pipetting, we recommend using pink colored TMB ONE Prestained (cat. no. 4430). The pink indicator disappears after addition of the stop solution.

The product is available in bulk quantities and as customized solutions.