TMB ONE Accessory Reagent
Référence OOSA09597
Conditionnement : 100ml
Marque : Aviva Systems Biology
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TMB ONE Accessory Reagent (OOSA09597)
| Datasheets/Manuals | Printable datasheet for TMB ONE Accessory Reagent (OOSA09597) |
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| Product Format | Liquid |
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| Application | ELISA |
| Reconstitution and Storage | 2°C to 8°C |
| Application Info | ELISA: Neat |
| Procedure | 1. Pour the desired amount of OOSA09597 into a sealable container and allow it to reach room temperature in the dark. Note that 0.1ml will be required per well. 2. Add 0.1ml of solution per microtiter well. 3. Allow development of OOSA09597. Development time is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation. i.e. wrapped in tinfoil. 4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour. 5. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of OOSA09597 is not recommended). |
| Description of Target | OOSA09597 is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP). TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. OOSA09597 produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm. |
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