TMB PLUS2 Accessory Reagent
Référence OOSA09610
Conditionnement : 500ml
Marque : Aviva Systems Biology
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TMB PLUS2 Accessory Reagent (OOSA09610)
| Datasheets/Manuals | Printable datasheet for TMB PLUS2 Accessory Reagent (OOSA09610) |
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| Product Format | Liquid |
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| Application | ELISA |
| Additional Information | Instructions for use: 1.) It is recommended that 100ul of TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark. 2.) Add 100ul substrate solution per microtiter well. 3.) Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil. 4.) For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour. N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of this product is not recommended). |
| Reconstitution and Storage | 2°C to 8°C |
| Application Info | ELISA: Neat |
| Intended Use | This product is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP).This product contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development. This product produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm. |
| Description of Target | TMB PLUS2 |
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