TNF alpha (Tumor Necrosis Factor alpha, TNFA, DIF, TNLG1F) (Biotin)

TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow Cytometry: Intracellular staining.
ELISA (detection): in combination with capture antibody 046195
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.