TNR siRNA (Human)
Référence MBS827617-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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TNR siRNA – Tenascin-R (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827617MBS827617 is an siRNA targeting TNR. Also known as TNR siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit TNR expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
TNR, siRNA
Full Product Name
TNR siRNA (Human)
Product Gene Name
TNR sirna
Product Synonym Names
Tenascin-R; TN-R; Janusin; Restrictin
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
TNR siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
139,695 Da
OMIM
601995
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TNR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for TNR sirna
RNA Interference (RNAi)
Other Notes
Small volumes of TNR sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TNR. It may not necessarily be applicable to this product.
NCBI Official Full Name
tenascin-R
NCBI Official Synonym Full Names
tenascin R
NCBI Official Symbol
TNR
NCBI Official Synonym Symbols
TN-R
UniProt Protein Name
Tenascin-R
UniProt Synonym Protein Names
Janusin; Restrictin
Protein Family
Tenascin
UniProt Gene Name
TNR
UniProt Synonym Gene Names
TN-R
UniProt Entry Name
TENR_HUMAN
NCBI Protein Information
tenascin-R
NCBI Summary for TNR
This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets. [provided by RefSeq, Aug 2013]
Show more UniProt Comments for TNR
TNR: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. Belongs to the tenascin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q24
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region; nucleus; lipid raft
Biological Process: negative regulation of axon extension involved in regeneration; axon guidance; extracellular matrix organization and biogenesis; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission; synapse organization and biogenesis; neuron adhesion; negative regulation of cell-cell adhesion; cell adhesion; neuromuscular process controlling balance; associative learning; telencephalon cell migration; positive regulation of transmission of nerve impulse
Show more Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q24
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region; nucleus; lipid raft
Biological Process: negative regulation of axon extension involved in regeneration; axon guidance; extracellular matrix organization and biogenesis; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission; synapse organization and biogenesis; neuron adhesion; negative regulation of cell-cell adhesion; cell adhesion; neuromuscular process controlling balance; associative learning; telencephalon cell migration; positive regulation of transmission of nerve impulse
Research Articles on TNR
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TNR sirna
| Reference Product | PubMed Publications |
|---|---|
| BCAN sirna | >2 publications with TNR and BCAN |
| NCAN sirna | >1 publications with TNR and NCAN |
| VCAN sirna | >1 publications with TNR and VCAN |
Pathways associated with TNR sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| ECM Proteoglycans Pathway antibodies | ECM Proteoglycans Pathway Diagram |
| ECM-receptor Interaction Pathway antibodies | ECM-receptor Interaction Pathway Diagram |
| ECM-receptor Interaction Pathway antibodies | ECM-receptor Interaction Pathway Diagram |
| Extracellular Matrix Organization Pathway antibodies | Extracellular Matrix Organization Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| Focal Adhesion Pathway antibodies | Focal Adhesion Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
| PI3K-Akt Signaling Pathway antibodies | PI3K-Akt Signaling Pathway Diagram |
Diseases associated with TNR sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >45 publications with TNR and Nervous System Diseases |
| Carcinoma Antibodies | >20 publications with TNR and Carcinoma |
| Neurologic Manifestations Antibodies | >16 publications with TNR and Neurologic Manifestations |
| Respiratory Tract Diseases Antibodies | >14 publications with TNR and Respiratory Tract Diseases |
| Liver Diseases Antibodies | >13 publications with TNR and Liver Diseases |
| Disease Models, Animal Antibodies | >13 publications with TNR and Disease Models, Animal |
| Necrosis Antibodies | >12 publications with TNR and Necrosis |
| Cognition Disorders Antibodies | >9 publications with TNR and Cognition Disorders |
| Cardiovascular Diseases Antibodies | >9 publications with TNR and Cardiovascular Diseases |
| Movement Disorders Antibodies | >8 publications with TNR and Movement Disorders |
Organs/Tissues associated with TNR sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >39 publications with TNR and Brain |
| Liver Antibodies | >22 publications with TNR and Liver |
| Testis Antibodies | >2 publications with TNR and Testis |
| Placenta Antibodies | >1 publications with TNR and Placenta |