TOP1 Protein, Human, Recombinant (His Tag)
Référence MBS8122098-0,1mg
Conditionnement : 0.1mg
Marque : MyBiosource
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TOP1 Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8122098MBS8122098 is a recombinant protein targeting TOP1. Also known as TOP1 Protein, Human, Recombinant (His Tag). Host: Human. More details
Product Overview
Online Datasheet in PDFProduct Name
TOP1, Recombinant Protein
Full Product Name
TOP1 Protein, Human, Recombinant (His Tag)
Product Gene Name
TOP1 recombinant protein
Product Synonym Names
Human TOP1 Protein (His Tag)
Sequence
Met1-Phe76 5
Host
Human
Form/Format
Supplied as sterile 20 mM Tris, 200 mM NaCl, 20% Glycerol, pH 7.5.
Purity/Purification
≥90% as determined by SDS-PAGE
Concentration
1.6 mg/mL (lot specific)
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Molecular Weight
Predicted MW: 93.1 kDa
Molecular Mass Note: The recombinant human TOP1 consists of 784 amino acids and predicts a molecular mass of 93.1 kDa
Molecular Mass Note: The recombinant human TOP1 consists of 784 amino acids and predicts a molecular mass of 93.1 kDa
Chromosome Location
Chromosome: 20; NC_000020.10 (39657462..39753127). Location: 20q12-q13.1
OMIM
126420
Source
Baculovirus-Insect Cells
Endotoxin
< 1.0 EU per ?g protein as determined by the LAL method
Predicted N Terminal
His
Protein Construction
A DNA sequence encoding the human TOP1 (NP_003277.1)(Met1-Phe765) was expressed with a polyhistidine tag at the N-terminus.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Other Notes
Small volumes of TOP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TOP1. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
UniProt Protein Name
DNA topoisomerase 1
UniProt Synonym Protein Names
DNA topoisomerase I
Protein Family
DNA topoisomerase
UniProt Gene Name
TOP1
UniProt Entry Name
TOP1_HUMAN
NCBI Protein Information
DNA topoisomerase 1; type I DNA topoisomerase
NCBI Summary for TOP1
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for TOP1
TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.
Protein type: Oncoprotein; Nucleolus; Isomerase; EC 5.99.1.2
Chromosomal Location of Human Ortholog: 20q12-q13.1
Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus
Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity
Biological Process: circadian rhythm; response to drug; chromatin remodeling; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation
Show more Protein type: Oncoprotein; Nucleolus; Isomerase; EC 5.99.1.2
Chromosomal Location of Human Ortholog: 20q12-q13.1
Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus
Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity
Biological Process: circadian rhythm; response to drug; chromatin remodeling; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with TOP1 recombinant protein
| Reference Product | PubMed Publications |
|---|---|
| TDP1 recombinant protein | >38 publications with TOP1 and TDP1 |
| JUN recombinant protein | >36 publications with TOP1 and JUN |
| PARP1 recombinant protein | >8 publications with TOP1 and PARP1 |
| TP53 recombinant protein | >4 publications with TOP1 and TP53 |
| CASP3 recombinant protein | >3 publications with TOP1 and CASP3 |
| SUMO1 recombinant protein | >2 publications with TOP1 and SUMO1 |
| BTBD2 recombinant protein | >2 publications with TOP1 and BTBD2 |
| BTBD1 recombinant protein | >2 publications with TOP1 and BTBD1 |
| TOPORS recombinant protein | >1 publications with TOP1 and TOPORS |
| CSNK2A1 recombinant protein | >1 publications with TOP1 and CSNK2A1 |
Pathways associated with TOP1 recombinant protein
| Products by Pathway | Pathway Diagram |
|---|---|
| Caspase Cascade In Apoptosis Pathway antibodies | Caspase Cascade In Apoptosis Pathway Diagram |
| Integrated Pancreatic Cancer Pathway antibodies | Integrated Pancreatic Cancer Pathway Diagram |
Diseases associated with TOP1 recombinant protein
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >129 publications with TOP1 and Neoplasms |
| Drug Toxicity Antibodies | >39 publications with TOP1 and Drug Toxicity |
| Skin Diseases Antibodies | >23 publications with TOP1 and Skin Diseases |
| Breast Neoplasms Antibodies | >21 publications with TOP1 and Breast Neoplasms |
| Nervous System Diseases Antibodies | >14 publications with TOP1 and Nervous System Diseases |
| Neoplasms, Experimental Antibodies | >14 publications with TOP1 and Neoplasms, Experimental |
| Adenocarcinoma Antibodies | >12 publications with TOP1 and Adenocarcinoma |
| Brain Diseases Antibodies | >9 publications with TOP1 and Brain Diseases |
| Melanoma Antibodies | >8 publications with TOP1 and Melanoma |
| Kidney Diseases Antibodies | >7 publications with TOP1 and Kidney Diseases |
Organs/Tissues associated with TOP1 recombinant protein
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >28 publications with TOP1 and Blood |
| Lung Antibodies | >20 publications with TOP1 and Lung |
| Prostate Antibodies | >13 publications with TOP1 and Prostate |
| Kidney Antibodies | >8 publications with TOP1 and Kidney |
| Brain Antibodies | >8 publications with TOP1 and Brain |
| Bone Antibodies | >7 publications with TOP1 and Bone |
| Bone Marrow Antibodies | >7 publications with TOP1 and Bone Marrow |
| Thymus Antibodies | >7 publications with TOP1 and Thymus |
| Ovary Antibodies | >5 publications with TOP1 and Ovary |
| Skin Antibodies | >5 publications with TOP1 and Skin |
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